Imae Y, Shinozaki K, Okazaki R
J Virol. 1976 Sep;19(3):765-74. doi: 10.1128/JVI.19.3.765-774.1976.
[3H]dTTP was not incorporated into T4 DNA in the in vitro system for T4 DNA replication when the system was prepared from cells infected with T4 amber mutants defective in DNA replication. [3H]dTTP incorporation was resumed by adding the missing gene product to the defective system. DNA replication by the reconstituted system proceeded by the discontinuous mode of replication, as observed in the wild-type system. By using this in vitro complementation system, molecular weights of gene 41, 43, 44, 45, and 62 products in the active form were roughly estimated as 60,000, 130,000, 130,000, 60,000, and 130,000, respectively. Complex formation between the products of genes 44 and 62 was detected. Other strong interactions between the gene products tested were not detected by glycerol density gradient sedimentation. Interaction of gene products with denatured DNA was analyzed by using a DNA-agarose column, and the results showed that products of genes 32 and 43 had a strong affinity for DNA.
当用感染了DNA复制缺陷型T4琥珀突变体的细胞制备体外T4 DNA复制系统时，[3H]dTTP未掺入该系统的T4 DNA中。通过向缺陷系统中添加缺失的基因产物，[3H]dTTP掺入得以恢复。重构系统进行的DNA复制以不连续复制模式进行，如同在野生型系统中观察到的那样。通过使用这种体外互补系统，活性形式的基因41、43、44、45和62产物的分子量大致分别估计为60,000、130,000、130,000、60,000和130,000。检测到了基因44和62产物之间的复合物形成。通过甘油密度梯度沉降未检测到所测试基因产物之间的其他强相互作用。利用DNA-琼脂糖柱分析了基因产物与变性DNA的相互作用，结果表明基因32和43的产物对DNA具有很强的亲和力。
