体外互补作为噬菌体T4 DNA复制所需新蛋白质的一种检测方法：T4基因44和62所指定复合物的纯化。
In vitro complementation as an assay for new proteins required for bacteriophage T4 DNA replication: purification of the complex specified by T4 genes 44 and 62.
作者信息
Barry J, Alberts B
出版信息
Proc Natl Acad Sci U S A. 1972 Sep;69(9):2717-21. doi: 10.1073/pnas.69.9.2717.
Abstract
We have developed an in vitro complementation assay for six T4 bacteriophage gene products believed to be components of the T4 DNA replication apparatus. This assay is based upon the fact that DNA synthesis in an infected cell lysate that lacks a given gene product is specifically stimulated by addition of the missing product. By the use of such an assay, two proteins that appear to be the products of T4 genes 44 and 62 have been purified to electrophoretic homogeneity as a single complex of the two polypeptide chains.
摘要
我们已经针对六种被认为是T4噬菌体DNA复制装置组成成分的T4噬菌体基因产物开发了一种体外互补测定法。该测定法基于这样一个事实，即缺乏特定基因产物的感染细胞裂解物中的DNA合成会因添加缺失的产物而受到特异性刺激。通过使用这种测定法，两种似乎是T4基因44和62产物的蛋白质已被纯化至电泳纯，成为由两条多肽链组成的单一复合物。
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In vitro complementation as an assay for new proteins required for bacteriophage T4 DNA replication: purification of the complex specified by T4 genes 44 and 62.体外互补作为噬菌体T4 DNA复制所需新蛋白质的一种检测方法：T4基因44和62所指定复合物的纯化。
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