Crowley J H, Tove S, Parks L W
Department of Microbiology, North Carolina State University, Raleigh, NC 27695-7615, USA.
Curr Genet. 1998 Aug;34(2):93-9. doi: 10.1007/s002940050371.
ERG24 is the structural gene for the C14-sterol reductase in yeast. A lack of activity in that enzyme, mediated either by the morpholine fungicides or the insertional inactivation of ERG24, causes the accumulation of the aberrant sterol ignosterol. Cells producing this sterol are unable to grow aerobically in the routine laboratory medium, YPD. However, growth does occur on a synthetic defined medium. A novel calcium-dependent phenotype associated with alterations in the ergosterol biosynthetic pathway in yeast is described. In addition, reduction of yeast growth with an azole inhibitor of the C-14 sterol de-methylase was also modulated by an excess of calcium ions in the culture medium. These results define a new effect of ergosterol deficiency and provide important practical implications for utilizing morpholine and azole sterol biosynthetic-inhibiting fungicides.
ERG24是酵母中C14 - 甾醇还原酶的结构基因。由吗啉类杀菌剂或ERG24的插入失活介导的该酶活性缺乏，会导致异常甾醇豆甾醇的积累。产生这种甾醇的细胞在常规实验室培养基YPD中无法进行有氧生长。然而，在合成限定培养基上确实会生长。本文描述了一种与酵母中麦角甾醇生物合成途径改变相关的新型钙依赖性表型。此外，培养基中过量的钙离子也调节了用C - 14甾醇脱甲基酶的唑类抑制剂对酵母生长的抑制作用。这些结果定义了麦角甾醇缺乏的新效应，并为利用吗啉和唑类甾醇生物合成抑制性杀菌剂提供了重要的实际意义。
