Burg M B, Green N
Kidney Int. 1976 Sep;10(3):221-8. doi: 10.1038/ki.1976.101.
Proximal convoluted tubules were dissected from rabbit kidneys and perfused in vitro in order to determine the effect of monovalent ions on fluid absorption and transepithelial voltage. Replacement of sodium in the perfusate and bath by lithium, tetramethyl ammonium or choline caused the rate of fluid absorption and voltage to fall to near zero. Replacement of potassium in the bath by sodium had the identical effect. Replacement of chloride by nitrate or perchlorate had comparatively little effect. The results are consistent with the generally held view that active sodium transport (mediated by a Na- and K- activated adenosine triphosphatase) is the primary process responsible for the absorption of the fluid and the voltage. Replacement of bicarbonate in the perfusate and bath by chloride caused the rate of fluid absorption to decrease by 33%. The possible relation between sodium transport and bicarbonate is discussed.
从兔肾中分离出近端曲管并在体外进行灌注，以确定单价离子对液体吸收和跨上皮电压的影响。用锂、四甲基铵或胆碱取代灌注液和浴液中的钠会导致液体吸收速率和电压降至接近零。用钠取代浴液中的钾也有相同的效果。用硝酸盐或高氯酸盐取代氯的影响相对较小。这些结果与普遍认为的观点一致，即活性钠转运（由钠钾激活的三磷酸腺苷酶介导）是负责液体吸收和电压的主要过程。用氯取代灌注液和浴液中的碳酸氢盐会使液体吸收速率降低33%。讨论了钠转运与碳酸氢盐之间的可能关系。
