Gortázar P, Roën A, Vázquez J T
Instituto Universitario de Bio-Orgánica Antonio González, Universidad de La Laguna, Tenerife, Spain.
Chirality. 1998;10(6):507-12. doi: 10.1002/(SICI)1520-636X(1998)10:6<507::AID-CHIR1>3.0.CO;2-0.
A study of the applicability of circular dichroism (CD) for the determination of drug levels in human serum is described and a new method for the quantitative determination of optically active absorbing drugs having Cotton effects at wavelengths about 250 nm in human serum and/or plasma is proposed. The principal advantages of this method are speed, economy, and simplicity, no derivatization or chromatographic separation steps being needed. The validity of the CD determination was confirmed by analysis of variance, beta-lactam antibiotics being chosen as model drugs. In addition, the validation studies performed confirm the accuracy and precision of the proposed method. For beta-lactam antibiotics lacking Cotton effects above 250 nm, an alternative method based on the extraction of the drug from serum is considered.
本文描述了一项关于圆二色性（CD）在测定人血清中药物水平适用性的研究，并提出了一种定量测定在约250nm波长处具有科顿效应的光学活性吸收性药物在人血清和/或血浆中含量的新方法。该方法的主要优点是快速、经济且简便，无需衍生化或色谱分离步骤。通过方差分析证实了CD测定的有效性，选择β-内酰胺类抗生素作为模型药物。此外，所进行的验证研究证实了该方法的准确性和精密度。对于在250nm以上缺乏科顿效应的β-内酰胺类抗生素，考虑了一种基于从血清中提取药物的替代方法。
