Gortázar P, Ravina M, Vázquez J T
Centro de Productos Naturales Orgánicos Antonio González, Universidad de La Laguna, Tenerife, Spain.
J Pharm Sci. 1995 Nov;84(11):1316-21. doi: 10.1002/jps.2600841111.
Over 200 urine samples from 61 subjects were analyzed by circular dichroism spectroscopy. The results proved that this technique can be applied to the direct determination of optically active absorbing drugs in urine of subjects under multiple drug administration, independently of the presence of proteins, which can simultaneously be determined. A list of noninterfering drugs is included. The validity of the present method was confirmed by analysis of variance, the beta-lactam antibiotics ampicillin, cefoxitin, and cephalexin being chosen as model drugs and human albumin as the analytical standard for protein determination. The results demonstrated that the proposed method is accurate and precise, the correlation coefficients being higher than 0.9996. A circular dichroism and HPLC data comparison was successfully performed. The principal advantages of this method are simplicity, quickness, and economy, no derivatization or chromatographic separation steps being needed.
对来自61名受试者的200多个尿液样本进行了圆二色光谱分析。结果证明，该技术可用于直接测定多药给药受试者尿液中的旋光吸收药物，不受蛋白质存在的影响，同时还能测定蛋白质。文中列出了无干扰药物清单。通过方差分析证实了本方法的有效性，选择β-内酰胺类抗生素氨苄西林、头孢西丁和头孢氨苄作为模型药物，以人白蛋白作为蛋白质测定的分析标准。结果表明，所提出的方法准确、精密，相关系数高于0.9996。成功地进行了圆二色光谱与高效液相色谱数据的比较。该方法的主要优点是简单、快速、经济，无需衍生化或色谱分离步骤。
