Yamasaki Y, Kuwata K, Hioki K, Sowa M, Hirakawa K, Okajima K, Isozaki H, Iwanaga T, Furukawa H, Nakano H, Watanabe A, Takami M, Tsukahara Y, Matsunaga S, Kikkawa N, Gotoh T, Tokuda H, Takahashi S, Toyohara M, Yagi M, Takebayashi J, Nakajima T, Miyazaki H, Kuwata H, Nakazato H
Dept. of Surgery, Osaka Kousei Nenkin Hospital.
Gan To Kagaku Ryoho. 1998 Aug;25(10):1549-56.
Pyrimidine nucleoside phosphorylase (PyNPase), among which thymidine phosphorylase (dThdPase) exists mainly in human tumor tissues, is an enzyme to convert 5'-deoxy-5-fluorouridine (5'-DFUR) to 5-fluorouracil. Recently, it was reported that dThdPase was identical to platelet-derived endothelial cell growth factor, angiogenetic factor. Therefore, we expect that there is possibility of dThdPase being a prognostic factor.
We investigated for a possible correlation between PyNPase activities in tumor tissues and prognostic factors of histological findings, examined the influences of preoperative oral 5'-DFUR administration to PyNPase levels and investigated for a correlation between HPLC methods and ELISA methods in patients with gastric cancer.
Higher levels of PyNPase were observed in patients with advanced t,n,v, and ly factors. PyNPase levels decreased by 5'-DFUR in patients with differentiated cases. A high correlation was found between HPLC and ELISA methods.
This study suggests that we must investigate possibility of PyNPase being a prognostic factor in more detail.
嘧啶核苷磷酸化酶（PyNPase），其中胸苷磷酸化酶（dThdPase）主要存在于人类肿瘤组织中，是一种将5'-脱氧-5-氟尿苷（5'-DFUR）转化为5-氟尿嘧啶的酶。最近，有报道称dThdPase与血小板衍生的内皮细胞生长因子、血管生成因子相同。因此，我们预期dThdPase有可能成为一种预后因素。
我们研究了肿瘤组织中PyNPase活性与组织学结果的预后因素之间可能存在的相关性，检测了术前口服5'-DFUR对PyNPase水平的影响，并研究了胃癌患者中高效液相色谱法（HPLC）与酶联免疫吸附测定法（ELISA）之间的相关性。
在具有高级别t、n、v和ly因子的患者中观察到较高水平的PyNPase。在分化型病例的患者中，PyNPase水平因5'-DFUR而降低。HPLC法与ELISA法之间存在高度相关性。
本研究表明，我们必须更详细地研究PyNPase作为预后因素的可能性。
