Otim O, Singhal R P
Department of Chemistry, Wichita State University, Kansas 67260-0051, USA.
J Capillary Electrophor. 1997 Sep-Oct;4(5):247-51.
The electrophoretic resolution (selectivity) of double-stranded DNA (dsDNA) restriction fragments is studied for different organisms (phi X174, lambda phage, and pGEM-3 plasmids) by various separation and detection methods. The selectivity can be predicted by plotting the difference in chain length over their average chain length (base pair difference/average base pair magnitude of delta bp/Ave.-bp) against each DNA fragment pair. Experimental conditions and the nature of the DNA can influence the predicted results.
