Guttman A
Beckman Instruments, Inc., Palo Alto, California 94304.
Appl Theor Electrophor. 1992;3(2):91-6.
The influence of operating conditions on the separation of double stranded DNA molecules in capillary polyacrylamide gel electrophoresis is presented in continuation of previous work. In this study, an equation was derived that relates the effects of electrophoresis variables such as gel concentration, field strength, molecular weight and temperature to the migration velocity. As a model system, phage phi X174 DNA restriction fragments obtained by digestion with HaeIII have been examined. As an illustration of optimized conditions, DNA molecules up to the 10(3) base pair range were separated at high temperature (50 degrees C) in less than 6 min and with efficiencies as high as 5000 plates m-1 s-1.
继先前的工作之后，本文介绍了操作条件对毛细管聚丙烯酰胺凝胶电泳中双链DNA分子分离的影响。在本研究中，推导了一个方程，该方程将凝胶浓度、场强、分子量和温度等电泳变量的影响与迁移速度联系起来。作为模型系统，研究了用HaeIII消化得到的噬菌体phi X174 DNA限制性片段。作为优化条件的一个示例，在高温（50摄氏度）下，不到6分钟就分离出了长达10³碱基对范围的DNA分子，效率高达5000塔板米⁻¹秒⁻¹。
