Dabas P C, Vescina M C, Carducci C N
Department of Analytical Chemistry and Physicochemistry, Faculty of Pharmacy and Biochemistry, University of Buenos Aires, Argentina.
J Capillary Electrophor. 1997 Sep-Oct;4(5):253-6.
A simple and reproducible micellar electrokinetic chromatography (MEKC) method has been developed for the quantitation of suramin serum levels to be used for its therapeutic drug monitoring (TDM). A running buffer solution of 20 mM sodium borate, 75 mM sodium dodecyl sulfate (SDS), and 4 M urea at pH 9.2 were employed and samples were introduced directly into the capillary. The voltage applied for sample separation was 25 kV and UV detection was at 254 nm. Linearity was proved over the range 47.6 micrograms/mL-523.6 micrograms/mL of suramin (r: 0.9996). Orange G was used as internal standard and no interferences of common drugs simultaneously administered to patients were observed. Recovery values of the intraday and interday assays were between 92.5 and 97.2%.
已开发出一种简单且可重复的胶束电动毛细管色谱法（MEKC），用于定量测定舒拉明血清水平，以用于其治疗药物监测（TDM）。采用pH 9.2的20 mM硼酸钠、75 mM十二烷基硫酸钠（SDS）和4 M尿素的运行缓冲溶液，将样品直接注入毛细管。用于样品分离的电压为25 kV，紫外检测波长为254 nm。舒拉明在47.6微克/毫升至523.6微克/毫升范围内呈线性（r：0.9996）。使用橙黄G作为内标，未观察到同时给予患者的常用药物的干扰。日内和日间测定的回收率在92.5%至97.2%之间。
