Giorgieri S, D'Antuono A, Pañak K, Ruiz O, Díaz L
Cátedra de Química Analítica Instrumental, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina.
J Capillary Electrophor. 1997 Nov-Dec;4(6):257-60.
A reproducible, rapid procedure for the determination of homocysteine, cysteine, and glutathione in human blood by micellar electrokinetic chromatography (MEKC) has been developed. Whole blood samples were deproteinized and centrifuged, and the supernatant was directly introduced into the capillary for analysis without further derivatization. Separations were performed using an uncoated capillary of 30 cm effective length and 50 microns i.d.; 20 mM phosphate, 20 mM borate, and 50 mM SDS, pH 9.00, as running buffer; and an applied voltage of 18 kV. On-line detection was carried out at 214 nm.
已开发出一种通过胶束电动色谱法（MEKC）测定人血中同型半胱氨酸、半胱氨酸和谷胱甘肽的可重现、快速的方法。全血样品进行去蛋白处理并离心，上清液直接注入毛细管中进行分析，无需进一步衍生化。使用有效长度为30 cm、内径为50微米的未涂层毛细管进行分离；以20 mM磷酸盐、20 mM硼酸盐和50 mM SDS（pH 9.00）作为运行缓冲液；施加电压为18 kV。在214 nm处进行在线检测。
