Krause W L, Kazemi H, Burton M D
Pulmonary and Critical Care Unit, Massachusetts General Hospital, Boston 02114, USA.
Neurosci Lett. 1998 Jul 31;251(3):153-6. doi: 10.1016/s0304-3940(98)00502-3.
We used the imidazole-binding agent, diethylpyrocarbonate (DEPC), to test the hypothesis that rhythmic respiratory activity of the in vitro neonatal rat brainstem-spinal cord preparation was functionally dependent on imidazole. Neural activity was recorded from spinal nerves (C1-C4) during superfusion with 95%O2/5%CO2 buffer at pH 7.3 and T = 26 degrees C. Superfusate containing DEPC (40 mM) caused cessation of rhythmic activity within minutes. In eight of 33 preparations, microinjection of DEPC (32 nmol) onto the ventral medullary surface (VMS) reduced burst amplitude by at least 50% within 10 min, and in 12 of 33 preparations, microinjection of DEPC produced neural apnea. Therefore, we conclude that proteins containing imidazole near the VMS are critically important for the maintenance of rhythmic respiratory activity in vitro. Furthermore, alphastat regulation of respiration may be an essential trait of this preparation.
我们使用咪唑结合剂焦碳酸二乙酯（DEPC）来验证体外新生大鼠脑干-脊髓标本的节律性呼吸活动在功能上依赖于咪唑这一假说。在pH 7.3、温度为26摄氏度的条件下，用95%O₂/5%CO₂缓冲液灌注时，记录脊髓神经（C1-C4）的神经活动。含有DEPC（40 mM）的灌注液在数分钟内导致节律性活动停止。在33个标本中的8个中，向延髓腹侧面（VMS）微量注射DEPC（32 nmol）在10分钟内使爆发幅度至少降低了50%，在33个标本中的12个中，微量注射DEPC导致神经呼吸暂停。因此，我们得出结论，VMS附近含咪唑的蛋白质对于体外维持节律性呼吸活动至关重要。此外，呼吸的α稳态调节可能是该标本的一个基本特征。
