Oberkofler H, Liu Y M, Esterbauer H, Hell E, Krempler F, Patsch W
Department of Laboratory Medicine, Landeskrankenanstalten Salzburg, Austria.
Diabetologia. 1998 Aug;41(8):940-6. doi: 10.1007/s001250051011.
The mitochondrial uncoupling protein-2 (UCP-2) is a recently discovered homologue of the brown adipose tissue-specific uncoupling protein and could be involved in the regulation of energy balance. Since obesity is associated with disturbed energy homeostasis, we tested the hypothesis that UCP-2 gene expression is deficient in this disorder. We determined, by a competitive reverse transcription-polymerase chain reaction assay, UCP-2 mRNA expression in intra- and extraperitoneal adipose tissues of 107 morbidly obese subjects and 31 lean control subjects. In both obese and non-obese subjects, UCP-2 mRNA abundance was higher in the intraperitoneal than in the extraperitoneal tissue (p < 0.05), but no association was observed between intra- and extraperitoneal expression in either group. Compared with lean control subjects, both male and female obese subjects displayed significantly lower average UCP-2 mRNA expression in the intraperitoneal adipose tissue (p < 0.006), while UCP-2 mRNA abundance in extraperitoneal adipose tissue was not different between obese and non-obese men and women. Intraperitoneal UCP-2 mRNA remained low in nine obese subjects who lost 23 +/- 12 kg of weight over a period of 10 +/- 5 months subsequent to weight reducing surgery. These data support the concept that impaired adipose tissue expression of UCP2 may play a role in the pathophysiology of obesity.
线粒体解偶联蛋白2（UCP - 2）是最近发现的棕色脂肪组织特异性解偶联蛋白的同源物，可能参与能量平衡的调节。由于肥胖与能量稳态紊乱有关，我们检验了UCP - 2基因表达在这种疾病中存在缺陷的假说。我们通过竞争性逆转录 - 聚合酶链反应分析，测定了107名病态肥胖受试者和31名瘦对照受试者腹膜内和腹膜外脂肪组织中UCP - 2 mRNA的表达。在肥胖和非肥胖受试者中，腹膜内UCP - 2 mRNA丰度均高于腹膜外组织（p < 0.05），但两组中腹膜内和腹膜外表达之间均未观察到相关性。与瘦对照受试者相比，肥胖男性和女性腹膜内脂肪组织中UCP - 2 mRNA的平均表达均显著降低（p < 0.006），而肥胖和非肥胖男性及女性腹膜外脂肪组织中UCP - 2 mRNA丰度无差异。在减肥手术后10±5个月内体重减轻23±12 kg的9名肥胖受试者中，腹膜内UCP - 2 mRNA水平仍然较低。这些数据支持了UCP2脂肪组织表达受损可能在肥胖病理生理学中起作用的观点。
