Boucabeille C, Letellier L, Simonet J M, Henckes G
Institut de Génétique et Microbiologie, Unité de Recherche Associée au Centre National de la Recherche Scientifique 2225, Université Paris-Sud, 91405 Orsay Cedex 05, France.
Appl Environ Microbiol. 1998 Sep;64(9):3416-21. doi: 10.1128/AEM.64.9.3416-3421.1998.
Linenscin OC2 is a small hydrophobic substance produced by the orange cheese coryneform bacterium Brevibacterium linens OC2. Linenscin OC2 inhibits growth of gram-negative bacteria with an altered outer membrane permeability and gram-positive bacteria. It is also able to lyse eucaryotic cells. The mode of action of linenscin OC2 on the Listeria innocua cytoplasmic membrane and the effects of environmental parameters were investigated. Addition of low doses of linenscin OC2 resulted in an immediate perturbation of the permeability properties of the cytoplasmic membrane and of the bacterial energetic state. Linenscin OC2 induced a loss of cytoplasmic potassium, depolarization of the cytoplasmic membrane, complete hydrolysis of internal ATP, efflux of inorganic phosphate, and transient increase in oxygen consumption. Potassium loss occurred in the absence of a proton motive force and was severely reduced at low temperatures, presumably as a result of increased ordering of the lipid hydrocarbon chains of the cytoplasmic membrane. We propose that linenscin OC2 interacts with the cytoplasmic membrane and that the permeability changes observed at low doses reflect the formation of pore-like structures in this membrane.
亚麻霉素OC2是由橙色奶酪棒状杆菌短杆菌属亚麻杆菌OC2产生的一种疏水性小分子物质。亚麻霉素OC2可抑制外膜通透性改变的革兰氏阴性菌和革兰氏阳性菌的生长。它还能够裂解真核细胞。研究了亚麻霉素OC2对无害李斯特菌细胞质膜的作用方式以及环境参数的影响。添加低剂量的亚麻霉素OC2会立即扰乱细胞质膜的通透性和细菌的能量状态。亚麻霉素OC2导致细胞质钾离子流失、细胞质膜去极化、内部ATP完全水解、无机磷酸盐外流以及氧气消耗短暂增加。钾离子流失在没有质子动力的情况下发生，并且在低温下严重减少，这可能是由于细胞质膜脂质烃链的有序性增加所致。我们认为亚麻霉素OC2与细胞质膜相互作用，并且在低剂量下观察到的通透性变化反映了该膜中孔状结构的形成。
