Ryser E T, Maisnier-Patin S, Gratadoux J J, Richard J
INRA, Station de Recherches Laitières, Jouy-En-Josas, France.
Int J Food Microbiol. 1994 Feb;21(3):237-46. doi: 10.1016/0168-1605(94)90030-2.
A newly developed hydrophobic grid membrane method was used to rapidly screen 105 traditional French cheeses for surface smear microorganisms inhibitory to Listeria monocytogenes strain V7. Approximately 125,000 colonies comprising a wide variety of bacteria were examined of which less than 0.1% produced visible zones of inhibition. Isolates possessing antilisterial activity consisted of various strains of Enterococcus faecalis, Staphylococcus xylosus, Staphylococcus warneri and coryneform bacteria, including one orange coryneform resembling Brevibacterium linens. All strains of E. faecalis and the orange coryneform that inhibited L. monocytogenes V7 exhibited strong inhibition against a panel of 21 Listeria strains comprised of L. monocytogenes (14 strains), L. innocua (two strains), L. ivanovii (two strains), L. seeligeri (two strains) and L. welshimeri (one strain). The remaining cheese isolates showed moderate to weak inhibition towards the same set of Listeria strains. Inhibitory substances produced by all strains except the orange coryneform were sensitive to one or more of five proteolytic enzymes tested and were therefore classified as bacteriocin-like inhibitory agents.
采用一种新开发的疏水网格膜法，对105种传统法国奶酪进行快速筛选，以寻找对单核细胞增生李斯特菌V7菌株有抑制作用的表面涂抹微生物。共检测了约125,000个包含多种细菌的菌落，其中产生可见抑菌圈的不到0.1%。具有抗李斯特菌活性的分离株包括粪肠球菌、木糖葡萄球菌、沃氏葡萄球菌的各种菌株以及棒状杆菌，其中一种橙色棒状杆菌类似于亚麻短杆菌。所有抑制单核细胞增生李斯特菌V7的粪肠球菌菌株和橙色棒状杆菌，对由14株单核细胞增生李斯特菌、2株无害李斯特菌、2株伊氏李斯特菌、2株斯氏李斯特菌和1株威氏李斯特菌组成的21株李斯特菌表现出强烈抑制作用。其余奶酪分离株对同一组李斯特菌菌株表现出中度至弱抑制作用。除橙色棒状杆菌外，所有菌株产生的抑菌物质对所测试的五种蛋白水解酶中的一种或多种敏感，因此被归类为类细菌素抑制因子。
