来自嗜碱芽孢杆菌的两种耐热碱性木聚糖酶的纯化及性质
Purification and properties of two thermostable alkaline xylanases from an alkaliphilic bacillus sp.
作者信息
Gessesse A
机构信息
Department of Biology, Addis Ababa University, Addis Ababa, Ethiopia.
出版信息
Appl Environ Microbiol. 1998 Sep;64(9):3533-5. doi: 10.1128/AEM.64.9.3533-3535.1998.
Abstract
Two xylanases, designated XylA and XylB, were purified from the culture supernatant of the alkaliphilic Bacillus sp. strain AR-009. The molecular masses of the two enzymes were estimated to be 23 kDa (XylA) and 48 kDa (XylB) by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The optimum pHs for activity were 9 for XylA and 9 to 10 for XylB. The temperature optima for the activity of XylA were 60 degreesC at pH 9 and 70 degreesC at pH 8. XylB was optimally active at 75 degreesC at pH 9 and 70 degreesC at pH 8. Both enzymes were stable in a broad pH range and showed good stability when incubated at 60 and 65 degreesC in pH 8 and 9 buffers.
摘要
从嗜碱芽孢杆菌AR-009菌株的培养上清液中纯化出两种木聚糖酶，分别命名为XylA和XylB。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳估计这两种酶的分子量分别为23 kDa（XylA）和48 kDa（XylB）。XylA的最佳活性pH值为9，XylB的最佳活性pH值为9至10。XylA在pH 9时活性的最佳温度为60℃，在pH 8时为70℃。XylB在pH 9时于75℃活性最佳，在pH 8时于70℃活性最佳。两种酶在较宽的pH范围内都很稳定，并且当在pH 8和9的缓冲液中于60℃和65℃孵育时表现出良好的稳定性。
相似文献
1
Purification and properties of two thermostable alkaline xylanases from an alkaliphilic bacillus sp.来自嗜碱芽孢杆菌的两种耐热碱性木聚糖酶的纯化及性质
Appl Environ Microbiol. 1998 Sep;64(9):3533-5. doi: 10.1128/AEM.64.9.3533-3535.1998.
2
Purification and Properties of Acid Stable Xylanases from Aspergillus kawachii.泡盛曲霉酸性稳定木聚糖酶的纯化及性质
Biosci Biotechnol Biochem. 1992 Jan;56(4):547-50. doi: 10.1271/bbb.56.547.
3
Purification and properties of a xylan-binding endoxylanase from Alkaliphilic bacillus sp. strain K-1.嗜碱芽孢杆菌K-1株木聚糖结合内切木聚糖酶的纯化及性质
Appl Environ Microbiol. 1999 Feb;65(2):694-7. doi: 10.1128/AEM.65.2.694-697.1999.
4
Purification and properties of the highly thermostable alkaline protease from an alkaliphilic and thermophilic Bacillus sp.嗜碱嗜热芽孢杆菌中高度耐热碱性蛋白酶的纯化及性质
J Biotechnol. 1993 Aug;30(2):245-56. doi: 10.1016/0168-1656(93)90117-6.
5
Purification and some properties of an alkaline xylanase from alkaliphilic Bacillus sp. strain 41M-1.嗜碱芽孢杆菌41M-1碱性木聚糖酶的纯化及某些性质
Appl Environ Microbiol. 1993 Jul;59(7):2311-6. doi: 10.1128/aem.59.7.2311-2316.1993.
6
Enzymatic properties of a novel liquefying alpha-amylase from an alkaliphilic Bacillus isolate and entire nucleotide and amino acid sequences.一株嗜碱芽孢杆菌分离株中新型液化α-淀粉酶的酶学性质、完整核苷酸序列及氨基酸序列
Appl Environ Microbiol. 1998 Sep;64(9):3282-9. doi: 10.1128/AEM.64.9.3282-3289.1998.
7
Production of alkaline xylanase by a newly isolated alkaliphilic Bacillus sp. strain 41M-1.一株新分离的嗜碱芽孢杆菌 41M-1 产碱性木聚糖酶的研究。
World J Microbiol Biotechnol. 1993 Mar;9(2):221-4. doi: 10.1007/BF00327842.
8
Highly thermostable and alkaline α-amylase from a halotolerant-alkaliphilic Bacillus sp. AB68.来自耐盐碱性芽孢杆菌 AB68 的高热稳定性和碱性 α-淀粉酶。
Braz J Microbiol. 2008 Jul;39(3):547-53. doi: 10.1590/S1517-838220080003000027. Epub 2008 Sep 1.
9
Purification and characterization of thermostable chitinase from Bacillus licheniformis SK-1.地衣芽孢杆菌SK-1中耐热几丁质酶的纯化与特性分析
Appl Biochem Biotechnol. 2009 Apr;157(1):23-35. doi: 10.1007/s12010-008-8328-7. Epub 2009 Feb 4.
10
Mutational analysis of endoxylanases XylA and XylB from the phytopathogen Fusarium graminearum reveals comprehensive insights into their inhibitor insensitivity.对植物病原菌禾谷镰刀菌的内切木聚糖酶XylA和XylB进行突变分析，揭示了它们对抑制剂不敏感的全面见解。
Appl Environ Microbiol. 2007 Jul;73(14):4602-8. doi: 10.1128/AEM.00442-07. Epub 2007 May 18.
引用本文的文献
1
A thermostable organic solvent-tolerant lipase from sp.: production and integrated downstream processing using an alcohol-salt-based aqueous two-phase system.来自嗜热栖热放线菌的一种耐热且耐有机溶剂脂肪酶：使用基于醇 - 盐的双水相系统进行生产及集成下游加工
Front Microbiol. 2023 Oct 13;14:1270270. doi: 10.3389/fmicb.2023.1270270. eCollection 2023.
2
Design and directed evolution of noncanonical β-stereoselective metalloglycosidases.非经典β-立体选择性金属糖苷酶的设计和定向进化。
Nat Commun. 2022 Nov 11;13(1):6844. doi: 10.1038/s41467-022-34713-8.
3
Multifactorial level of extremostability of proteins: can they be exploited for protein engineering?蛋白质极端稳定性的多因素水平：它们能否用于蛋白质工程？
Extremophiles. 2017 May;21(3):419-444. doi: 10.1007/s00792-016-0908-9. Epub 2017 Mar 10.
4
A high-molecular-weight, alkaline, and thermostable β-1,4-xylanase of a subseafloor Microcella alkaliphila.一种来自海底嗜碱微细胞菌的高分子量、碱性且耐热的β-1,4-木聚糖酶。
Extremophiles. 2016 Jul;20(4):471-8. doi: 10.1007/s00792-016-0837-7. Epub 2016 May 30.
5
Thermostable microbial xylanases for pulp and paper industries: trends, applications and further perspectives.用于制浆造纸工业的热稳定微生物木聚糖酶：趋势、应用及未来展望
World J Microbiol Biotechnol. 2016 Feb;32(2):34. doi: 10.1007/s11274-015-2005-0. Epub 2016 Jan 11.
6
Properties and Application of a Partially Purified Thermoalkali Stable Xylanase from Cellulosimicrobium sp. MTCC 10645 in Kraft Pulp Bleaching.来自纤维微杆菌属MTCC 10645的部分纯化的热碱稳定木聚糖酶在硫酸盐浆漂白中的性质及应用
ISRN Biotechnol. 2012 Jun 4;2013:872325. doi: 10.5402/2013/872325. eCollection 2013.
7
Properties of an alkali-thermo stable xylanase from Geobacillus thermodenitrificans A333 and applicability in xylooligosaccharides generation.嗜热脱氮地芽孢杆菌A333来源的一种碱热稳定木聚糖酶的性质及其在低聚木糖生成中的应用
World J Microbiol Biotechnol. 2015 Apr;31(4):633-48. doi: 10.1007/s11274-015-1818-1. Epub 2015 Feb 17.
8
High phylogenetic diversity of glycosyl hydrolase family 10 and 11 xylanases in the sediment of Lake Dabusu in China.中国大布苏湖沉积物中糖基水解酶家族10和11木聚糖酶的高系统发育多样性。
PLoS One. 2014 Nov 13;9(11):e112798. doi: 10.1371/journal.pone.0112798. eCollection 2014.
9
High secretory production of an alkaliphilic actinomycete xylanase and functional roles of some important residues.嗜碱放线菌木聚糖酶的高分泌生产及一些重要残基的功能作用
World J Microbiol Biotechnol. 2014 Jul;30(7):2053-62. doi: 10.1007/s11274-014-1630-3. Epub 2014 Mar 11.
10
Molecular and biochemical characterization of a new alkaline active multidomain xylanase from alkaline wastewater sludge.从碱性废水污泥中分离到一种新型碱性活性多结构域木聚糖酶的分子和生化特性。
World J Microbiol Biotechnol. 2013 Feb;29(2):327-34. doi: 10.1007/s11274-012-1186-z. Epub 2012 Nov 2.
本文引用的文献
1
Purification and Characterization of Two Xylanases from Alkalophilic Cephalosporium sp. Strain RYM-202.从嗜碱性顶头孢霉 RYM-202 中纯化和表征两种木聚糖酶。
Appl Environ Microbiol. 1996 Sep;62(9):3480-2. doi: 10.1128/aem.62.9.3480-3482.1996.
2
Purification, Characterization, and Substrate Specificities of Multiple Xylanases from Streptomyces sp. Strain B-12-2.从链霉菌 B-12-2 中分离纯化、鉴定及底物特异性分析多种木聚糖酶。
Appl Environ Microbiol. 1994 Jul;60(7):2609-15. doi: 10.1128/aem.60.7.2609-2615.1994.
3
Purification and properties of xylanase A from alkali-tolerant Bacillus sp. strain BP-23.耐碱芽孢杆菌BP-23菌株木聚糖酶A的纯化及性质
Appl Environ Microbiol. 1995 Dec;61(12):4468-70. doi: 10.1128/aem.61.12.4468-4470.1995.
4
Purification and characterization of a thermostable xylanase from Bacillus stearothermophilus T-6.嗜热脂肪芽孢杆菌T-6中一种耐热木聚糖酶的纯化与特性分析
Appl Environ Microbiol. 1993 Jun;59(6):1725-30. doi: 10.1128/aem.59.6.1725-1730.1993.
5
Purification and some properties of an alkaline xylanase from alkaliphilic Bacillus sp. strain 41M-1.嗜碱芽孢杆菌41M-1碱性木聚糖酶的纯化及某些性质
Appl Environ Microbiol. 1993 Jul;59(7):2311-6. doi: 10.1128/aem.59.7.2311-2316.1993.
6
Multiplicity of beta-1,4-xylanase in microorganisms: functions and applications.微生物中β-1,4-木聚糖酶的多样性：功能与应用
Microbiol Rev. 1988 Sep;52(3):305-17. doi: 10.1128/mr.52.3.305-317.1988.
7
Purification and characterization of two types of alkaline serine proteases produced by an alkalophilic actinomycete.嗜碱放线菌产生的两种碱性丝氨酸蛋白酶的纯化与特性分析
J Appl Bacteriol. 1990 Oct;69(4):520-9. doi: 10.1111/j.1365-2672.1990.tb01544.x.
Zotero 插件