Peek R, van Gelderen B E, Bruinenberg M, Kijlstra A
Netherlands Ophthalmic Research Institute, Amsterdam.
Invest Ophthalmol Vis Sci. 1998 Sep;39(10):1782-8.
To isolate tissue-specific gene products that contribute to corneal integrity.
A cDNA library was constructed and differentially hybridized. Cornea-specific clones were purified and further characterized.
In this study cornea-specific gene products were isolated by differential cDNA hybridization. In addition to known cornea-specific gene products, a transcript was isolated coding for a protein homologous to the angiopoietins, a recently described family of (anti)angiogenic factors. Subsequently, the full cDNA was sequenced, and the identified open reading frame was named cornea-derived transcript 6 (CDT6). Similar to the angiopoietins, CDT6 contains a hydrophobic NH2-terminal sequence, a coiled-coil domain, and a COOH-terminal fibrinogenlike domain. Expression of CDT6 could be detected only in the cornea and not in several other adult human tissues. Within the cornea, expression of CDT6 is confined to the stromal layer.
The human cornea shows high-level expression of a gene product homologous to the (anti)angiogenic factors, the angiopoietins. This homology, together with stromal-specific expression, suggests that this factor may contribute to the avascularity of the human cornea.
分离有助于维持角膜完整性的组织特异性基因产物。
构建cDNA文库并进行差异杂交。纯化角膜特异性克隆并进一步鉴定其特征。
在本研究中，通过差异cDNA杂交分离出角膜特异性基因产物。除了已知的角膜特异性基因产物外，还分离出一种转录本，其编码的蛋白质与血管生成素同源，血管生成素是最近描述的一类（抗）血管生成因子。随后，对完整的cDNA进行测序，并将鉴定出的开放阅读框命名为角膜衍生转录本6（CDT6）。与血管生成素相似，CDT6包含一个疏水的NH2末端序列、一个卷曲螺旋结构域和一个COOH末端纤维蛋白原样结构域。仅在角膜中可检测到CDT6的表达，在其他几种成人组织中则未检测到。在角膜内，CDT6的表达局限于基质层。
人角膜中显示出与（抗）血管生成因子血管生成素同源的基因产物的高水平表达。这种同源性以及基质特异性表达表明，该因子可能有助于人角膜的无血管状态。
