Panajotova V
Research Institute for Pharmacy and Biochemistry, Prague, Czech Republic.
Physiol Res. 1997;46(2):113-8.
The aim of the study was to determine the effect of selective dopaminergic agents [(+/-)-SKF-81297 (D1 agonist), R(-)-2,10,11-trihydroxy-N-propyl-noraporphine (D2 agonist), pergolid (D agonist), R(+)-SCH-23390 (D1 antagonist), S(-)-eticlopride (D2 antagonist) and cis-(Z)-flupenthixol (D antagonist)] on cell-mediated immune response in vivo and in vitro and to verify the presence of dopamine receptors on murine splenocytes. The tested dopaminergic compounds exhibited a pronounced inhibitory effect on T-dependent immunity. They suppressed alloantigen-induced immune response in vivo and in vitro, IL-2 production was also markedly reduced. No substantial difference was found between the effect of dopamine agonists and antagonists or among ligands of subtypes of dopamine receptors. The effect of dopaminergic agents in vitro indicates a direct interaction with immunocompetent cells at the peripheral level. As the binding studies did not confirm the presence of dopamine receptors on splenocytes, the immunosuppressive efficacy of dopaminergic agents does not seem to be mediated via specific dopamine receptors.
本研究的目的是确定选择性多巴胺能药物[（±）-SKF-81297（D1激动剂）、R（-）-2,10,11-三羟基-N-丙基去甲阿朴啡（D2激动剂）、培高利特（D激动剂）、R（+）-SCH-23390（D1拮抗剂）、S（-）-乙磺必利（D2拮抗剂）和顺式（Z）-氟哌噻吨（D拮抗剂）]对体内和体外细胞介导的免疫反应的影响，并验证小鼠脾细胞上多巴胺受体的存在。所测试的多巴胺能化合物对T细胞依赖性免疫表现出明显的抑制作用。它们在体内和体外均抑制同种异体抗原诱导的免疫反应，白细胞介素-2的产生也明显减少。多巴胺激动剂和拮抗剂的作用之间或多巴胺受体亚型的配体之间未发现实质性差异。多巴胺能药物在体外的作用表明其在外周水平与免疫活性细胞直接相互作用。由于结合研究未证实脾细胞上存在多巴胺受体，多巴胺能药物的免疫抑制作用似乎不是通过特异性多巴胺受体介导的。
