采用脉冲场凝胶电泳对日本临床来源和食品来源的单核细胞增生李斯特菌4b血清型分离株进行比较。
A comparison of Listeria monocytogenes serovar 4b isolates of clinical and food origin in Japan by pulsed-field gel electrophoresis.
作者信息
Nakama A, Terao M, Kokubo Y, Itoh T, Maruyama T, Kaneuchi C, McLauchlin J
机构信息
The Tokyo Metropolitan Research Laboratory of Public Health, Japan.
出版信息
Int J Food Microbiol. 1998 Jul 21;42(3):201-6. doi: 10.1016/s0168-1605(98)00084-1.
Pulsed-field gel electrophoresis (PFGE) patterns of 102 L. monocytogenes serovar 4b isolates from patients and foods examined in Japan were compared with 16 isolates from foodborne listeriosis episodes which occurred in North America or Europe. Using a combination of PFGE patterns with the restriction enzymes SmaI, ApaI, AscI and Sse8387I, 82 clinical isolates from Japan were categorized into 45 PFGE types: the largest group of 17 isolates (20.7%) were of the same PFGE type as cultures from the large foodborne outbreaks which occurred in California (1985) and Switzerland (1983-1987). Twenty cultures from foods on retail sale in Japan were classified into 12 PFGE types: four isolates were of three PFGE types also recognized among isolates of clinical origin from Japan, including the predominant clinical type.
将日本检测的来自患者和食品的102株单核细胞增生李斯特菌4b血清型菌株的脉冲场凝胶电泳（PFGE）图谱，与北美或欧洲发生的食源性李斯特菌病疫情中的16株菌株进行了比较。使用限制性内切酶SmaI、ApaI、AscI和Sse8387I组合的PFGE图谱，来自日本的82株临床分离株被分为45种PFGE类型：最大的一组17株（20.7%）与加利福尼亚州（1985年）和瑞士（1983 - 1987年）发生的大型食源性暴发中的培养物具有相同的PFGE类型。来自日本零售食品的20株培养物被分为12种PFGE类型：4株分离株属于在日本临床来源的分离株中也被识别出的3种PFGE类型，包括主要的临床类型。
Zotero 插件