Manzano M, Cocolin L, Cantoni C, Comi G
Dipartimento di Scienze degli Alimenti, Facoltà di Agraria, Università di Udine, Italy.
Int J Food Microbiol. 1998 Jul 21;42(3):207-12. doi: 10.1016/s0168-1605(98)00086-5.
Two highly specific primers for Listeria monocytogenes were used to yield from foods such as milk, soft cheese and meat, PCR products that were cleaved with the restriction enzyme HindIII. The fragments generated allowed a distinction between two groups of L. monocytogenes serovars: serovars 1/2a and 1/2c cluster in one group and serovars 1/2b, 3b and 4b in the other subgroup. Since this procedure can be completed in 24 h, an epidemiological association between human disease and suspected sources can be rapidly confirmed at the subgroup level in the laboratory.
使用针对单核细胞增生李斯特菌的两种高度特异性引物，从牛奶、软奶酪和肉类等食品中扩增出PCR产物，并用限制性内切酶HindIII进行切割。产生的片段可区分单核细胞增生李斯特菌血清型的两组：血清型1/2a和1/2c聚为一组，血清型1/2b、3b和4b聚为另一亚组。由于该过程可在24小时内完成，因此在实验室中可迅速在亚组水平确认人类疾病与疑似来源之间的流行病学关联。
