Sant'Angelo D B, Lucas B, Waterbury P G, Cohen B, Brabb T, Goverman J, Germain R N, Janeway C A
Section of Immunology, Yale University School of Medicine, Howard Hughes Medical Institute, New Haven, Connecticut 06520-8011, USA.
Immunity. 1998 Aug;9(2):179-86. doi: 10.1016/s1074-7613(00)80600-7.
Using a sensitive molecular marker for positive selection, the appearance of a particular functional TCR alpha chain sequence in cells from mice bearing a transgenic beta chain, we address several aspects of intrathymic T cell development. First, by examining specific TCR prior to and after maturation, we demonstrate how a restricted TCR repertoire is positively selected from a highly diverse immature TCR repertoire. Second, since this molecular marker is enriched in cells progressing toward the CD4 lineage and depleted in cells progressing toward the CD8 lineage, a map of the developmental pathway of alphabeta thymocytes can be inferred. Third, the first cells that show clear signs of positive intrathymic selection are identified.
利用一种用于阳性选择的灵敏分子标记，即在携带转基因β链的小鼠细胞中特定功能性TCRα链序列的出现，我们研究了胸腺内T细胞发育的几个方面。首先，通过检查成熟前后的特异性TCR，我们证明了如何从高度多样化的未成熟TCR库中阳性选择出受限的TCR库。其次，由于这种分子标记在向CD4谱系发展的细胞中富集，而在向CD8谱系发展的细胞中减少，因此可以推断出αβ胸腺细胞发育途径的图谱。第三，鉴定出了显示胸腺内阳性选择明确迹象的首批细胞。
