Hicks R R, Zhang L, Dhillon H S, Prasad M R, Seroogy K B
Department of Clinical Sciences, University of Kentucky, Lexington, KY 40536-0003, USA.
Brain Res Mol Brain Res. 1998 Aug 31;59(2):264-8. doi: 10.1016/s0169-328x(98)00158-2.
Recent investigations have shown that expression of mRNAs for the neurotrophins brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) is differentially altered in the hippocampus following traumatic brain injury. In the present study, modulation of neurotrophin receptor expression was examined in the hippocampus in a rat model of traumatic brain injury using in situ hybridization. Messenger RNA for trkB, the high-affinity receptor for BDNF and neurotrophin-4 (NT-4), was increased between 3 and 6 h bilaterally in the dentate gyrus following a lateral fluid-percussion brain injury of moderate severity (2.0-2.1 atm). No time-dependent alterations were observed for trkB mRNA in hippocampal subfields CA1 and CA3. Levels of mRNA for trkC, the high-affinity receptor for NT-3, did not change in any region of the hippocampus. These data demonstrate that lateral fluid-percussion injury modulates expression of trkB mRNA in the hippocampus and support a role for BDNF/trkB signalling mechanisms in secondary events associated with traumatic brain injury.
最近的研究表明，在创伤性脑损伤后，海马体中神经营养因子脑源性神经营养因子（BDNF）和神经营养因子-3（NT-3）的mRNA表达发生了不同程度的改变。在本研究中，使用原位杂交技术，在创伤性脑损伤大鼠模型的海马体中检测了神经营养因子受体表达的调节情况。在中度严重程度（2.0 - 2.1个大气压）的侧方流体冲击性脑损伤后，齿状回中BDNF和神经营养因子-4（NT-4）的高亲和力受体trkB的信使核糖核酸在双侧3至6小时内增加。在海马体亚区CA1和CA3中，未观察到trkB mRNA的时间依赖性变化。NT-3的高亲和力受体trkC的mRNA水平在海马体的任何区域均未发生变化。这些数据表明，侧方流体冲击性损伤可调节海马体中trkB mRNA的表达，并支持BDNF/trkB信号传导机制在与创伤性脑损伤相关的继发性事件中的作用。
