Kumar J P, Tio M, Hsiung F, Akopyan S, Gabay L, Seger R, Shilo B Z, Moses K
Department of Cell Biology, Emory University School of Medicine, Atlanta, GA 30322-3030, USA.
Development. 1998 Oct;125(19):3875-85. doi: 10.1242/dev.125.19.3875.
A new conditional Egfr allele was used to dissect the roles of the receptor in eye development and to test two published models. EGFR function is necessary for morphogenetic furrow initiation, is not required for establishment of the founder R8 cell in each ommatidium, but is necessary to maintain its differentiated state. EGFR is required subsequently for recruitment of all other neuronal cells. The initial EGFR-dependent MAP kinase activation occurs in the furrow, but the active kinase (dp-ERK) is observed only in the cytoplasm for over 2 hours. Similarly, SEVENLESS-dependent activation results in cytoplasmic appearance of dp-ERK for 6 hours. These results suggest an additional regulated step in this pathway and we discuss models for this.
一个新的条件性Egfr等位基因被用于剖析该受体在眼睛发育中的作用，并测试两个已发表的模型。EGFR功能对于形态发生沟的起始是必需的，在每个小眼的奠基性R8细胞的建立过程中并非必需，但对于维持其分化状态是必需的。随后，EGFR对于招募所有其他神经细胞是必需的。最初的EGFR依赖性丝裂原活化蛋白激酶激活发生在沟中，但活性激酶（dp-ERK）在超过2小时内仅在细胞质中被观察到。同样，SEVENLESS依赖性激活导致dp-ERK在细胞质中出现6小时。这些结果表明该信号通路中存在一个额外的调控步骤，我们对此进行了模型讨论。
