Ljunggren G, Anderson D J
Department of Obstetrics, Gynecology and Reproductive Biology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
J Reprod Immunol. 1998 Jul;38(2):123-38. doi: 10.1016/s0165-0378(98)00009-6.
Our purpose was to determine the expression of the major histocompatibility complex (MHC) class I and class II gene products as well as the costimulatory molecules B7-1 and B7-2 on cervical epithelial cells, and to determine to what extent inflammatory cytokines regulate their expression. Immunohistology and flow cytometry techniques were used to identify and quantify MHC class I and class II molecules, and the costimulatory molecules B7.1 and B7.2, on sections and primary epithelial cell cultures of human endo- and ectocervix. MHC class I but not class II molecules were constitutively expressed on tissue sections and primary epithelial cell cultures derived from endo- and ectocervix. Expression of MHC class I and class II was upregulated in vitro by IFN-gamma in a time and dose dependent fashion. The induction of class II expression was more pronounced on ectocervical cells than on endocervical cells. MHC class I but not class II expression was also enhanced by IFN-alpha as well as TNF-alpha. TNF-alpha and TGF-beta1 inhibited the IFN-gamma induced MHC class II expression. Expression of the costimulatory molecules B7-1 and B7-2 were not detected in tissue sections or on resting or cytokine-treated cervical epithelial cells in vitro. The present results support the concept that endo- and ectocervical epithelial cells, like their counterparts at other mucosal sites. constitutively express MHC class I molecules and can express MHC class II upon cytokine stimulation, indicating that they are capable of presenting antigens to T-cells.
我们的目的是确定主要组织相容性复合体（MHC）I类和II类基因产物以及共刺激分子B7-1和B7-2在宫颈上皮细胞上的表达，并确定炎性细胞因子在多大程度上调节它们的表达。采用免疫组织学和流式细胞术技术，对人子宫颈内膜和外膜的切片及原代上皮细胞培养物中的MHC I类和II类分子以及共刺激分子B7.1和B7.2进行鉴定和定量。MHC I类而非II类分子在源自子宫颈内膜和外膜的组织切片及原代上皮细胞培养物中组成性表达。体外，I型干扰素（IFN-γ）能以时间和剂量依赖的方式上调MHC I类和II类分子的表达。II类分子表达的诱导在外宫颈细胞上比在内宫颈细胞上更明显。I型干扰素（IFN-α）以及肿瘤坏死因子-α（TNF-α）也能增强MHC I类而非II类分子的表达。TNF-α和转化生长因子-β1（TGF-β1）抑制IFN-γ诱导的MHC II类分子表达。在组织切片中或体外静息或经细胞因子处理的宫颈上皮细胞上未检测到共刺激分子B7-1和B7-2的表达。目前的结果支持这样一种概念，即子宫颈内膜和外膜上皮细胞与其在其他黏膜部位的对应细胞一样，组成性表达MHC I类分子，并在细胞因子刺激下能够表达MHC II类分子，这表明它们有能力将抗原呈递给T细胞。
