Huniche B S, Jensen L T, Birkelund S, Christiansen G
Department of Medical Microbiology and Immunology, University of Aarhus, Denmark.
Scand J Infect Dis. 1998;30(2):181-7. doi: 10.1080/003655498750003609.
We examined 6 C. pneumonia isolates from The American Type Culture Collection (ATCC) and 2 Finnish isolates for Mycoplasma contamination. Three of the ATCC isolates and both of the Finnish isolates were Mycoplasma-contaminated. The contaminants were characterized by means of growth in BEa and BEg media, immunoblotting, polymerase chain reaction and pulsed field gel electrophoresis. Two of the 6 ATCC isolates [ATCC VR1355 (TWAR strain 2043) and ATCC VR1356 (TWAR strain 2023)] were infected with Mycoplasma hominis and 1 isolate [ATCC VR2282 (TWAR strain TW183)] was contaminated with both Mycoplasma hominis and Mycoplasma orale, whereas 3 of the ATCC isolates [ATCC VR1310, ATCC VR1360 (TWAR strain CM-1) and ATCC 53592 (TWAR strain AR39)] were not contaminated. The Finnish C. pneumoniae isolates Kajaani 6 and Parola were found to be contaminated with M. hominis and M. orale, respectively. The contamination of C. pneumoniae stock cultures, frequently used in the microimmunofluorescence test, with human pathogens, could pose a serious problem in C. pneumoniae serology.
我们检测了来自美国模式培养物集存库（ATCC）的6株肺炎衣原体分离株和2株芬兰分离株是否存在支原体污染。ATCC的3株分离株和芬兰的2株分离株均被支原体污染。通过在BEa和BEg培养基中生长、免疫印迹、聚合酶链反应和脉冲场凝胶电泳对污染物进行了鉴定。6株ATCC分离株中的2株[ATCC VR1355（TWAR菌株2043）和ATCC VR1356（TWAR菌株2023）]感染了人型支原体，1株分离株[ATCC VR2282（TWAR菌株TW183）]同时被人型支原体和口腔支原体污染，而ATCC的3株分离株[ATCC VR1310、ATCC VR1360（TWAR菌株CM - 1）和ATCC 53592（TWAR菌株AR39）]未被污染。芬兰的肺炎衣原体分离株Kajaani 6和Parola分别被发现感染了人型支原体和口腔支原体。常用于微量免疫荧光试验的肺炎衣原体储备培养物被人类病原体污染，这可能在肺炎衣原体血清学中造成严重问题。
