Matsushima Y, Nagabukuro A, Matsuda Y, Kitagawa Y
Biochemical Regulation, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Japan.
Cytogenet Cell Genet. 1998;81(3-4):194-8. doi: 10.1159/000015028.
Matrin 3 forms a family of nuclear proteins together with NP220s. Using cDNA sequences encoding rat matrin 3 as a probe, we isolated genomic clones of mouse matrin 3 gene and its two pseudogenes. The genuine mouse matrin 3 gene has an exon covering the N-terminal one third of matrin 3 with a sequence 99% identical to rat matrin 3 cDNA. The gene was localized to Chromosome 18C by in situ hybridization and mapped at 3.6 cM distal to D18Mit117 and 2.2 cM proximal to D18Mit14 on mouse Chromosome 18 by interspecific backcross analysis. One pseudogene has an exon-like sequence covering the N-terminal half of matrin 3 that is interrupted by an unknown sequence. Reverse transcription polymerase chain reaction (RT-PCR) of RNA from mouse liver and brain using unique sequences of the genuine gene and the pseudogene confirmed the expression of only the former. The other pseudogene has a sequence only 80% identical to rat cDNA and is partially deleted and reversed. These pseudogenes were localized to Chromosome 4E2 and 8D2, respectively.
Matrin 3与NP220s共同构成一个核蛋白家族。我们以编码大鼠Matrin 3的cDNA序列为探针，分离出了小鼠Matrin 3基因及其两个假基因的基因组克隆。真正的小鼠Matrin 3基因有一个外显子，覆盖Matrin 3的N端三分之一，其序列与大鼠Matrin 3 cDNA有99%的同一性。通过原位杂交将该基因定位到18号染色体C区，并通过种间回交分析将其定位在小鼠18号染色体上D18Mit117远端3.6 cM处和D18Mit14近端2.2 cM处。一个假基因有一个类似外显子的序列，覆盖Matrin 3的N端一半，被一个未知序列打断。使用真正基因和假基因的独特序列对小鼠肝脏和大脑的RNA进行逆转录聚合酶链反应（RT-PCR），证实只有前者表达。另一个假基因的序列与大鼠cDNA只有80%的同一性，并且部分缺失和颠倒。这些假基因分别定位到4号染色体E2区和8号染色体D2区。
