Shadmehri Mohammad, Ashrafi-Helan Javad, Firouzamandi Masoumeh
Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran.
Vet Res Forum. 2022 Sep;13(3):349-356. doi: 10.30466/vrf.2021.128764.2976. Epub 2022 Sep 15.
Ovine pulmonary adenocarcinoma (OPA) is a model of human lung cancer and fatal viral disease that causes neoplasia in sheep respiratory cells. In the current study, 986 lung samples was inspected in the slaughterhouse, and finally twenty OPA lung organs were clinically diagnosed and five healthy lung organs were assigned as the control sample. Three SSCP patterns were detected for the affected lungs animals in comparison with the healthy lungs. In addition, sequencing results indicated three different single point mutations in exon 4 of TP53 within infected lungs, whereas no mutations were observed in exon 9 of this gene. Real-time PCR results showed up-regulation of the TP53 gene in all the infected lung cells compared to healthy cells. There was significant correlation between the mutations in exon 4 and OPAand can be used as a useful tool in determining the mechanism of lung cancer.
绵羊肺腺癌（OPA）是一种人类肺癌和致命病毒性疾病的模型，可导致绵羊呼吸道细胞发生肿瘤形成。在本研究中，在屠宰场检查了986份肺样本，最终临床诊断出20个OPA肺器官，并将5个健康肺器官作为对照样本。与健康肺相比，在患病肺动物中检测到三种SSCP模式。此外，测序结果表明，在受感染的肺中，TP53基因外显子4存在三种不同的单点突变，而该基因的外显子9未观察到突变。实时PCR结果显示，与健康细胞相比，所有受感染的肺细胞中TP53基因均上调。外显子4中的突变与OPA之间存在显著相关性，可作为确定肺癌机制的有用工具。
