Espinosa L, Navarro E
UBCM-Institut Municipal d'Investigació Mèdica, Barcelona, Spain.
Cytogenet Cell Genet. 1998;81(3-4):278-82. doi: 10.1159/000015046.
The EMK (ELKL Motif Kinase) is a small family of ser/thr protein kinases involved in the control of cell polarity, microtubule stability and cancer. We have isolated several cDNA clones that encoded two isoforms of the human ser/thr protein kinase EMK1 (GDB symbol EMK1, alias ELKL motif kinase 1, and MARK2). These isoforms were characterized by the presence of a 162-bp alternative exon that gave rise to two forms, one containing the exon and the other one lacking it. Both forms were found to be coexpressed in a number of selected cell lines and tissue samples when analyzed by RT-PCR. By Northern blot, human EMK1 was shown to be encoded by a single mRNA ubiquitously expressed. We have reconstructed most of the genomic structure of EMK1 from the sequence of two human chromosome 11 cosmids deposited in databases, and showed that the gene is composed of a minimum of 16 small exons.
EMK（ELKL 基序激酶）是一个小的丝氨酸/苏氨酸蛋白激酶家族，参与细胞极性、微管稳定性和癌症的调控。我们已经分离出几个 cDNA 克隆，它们编码人丝氨酸/苏氨酸蛋白激酶 EMK1 的两种同工型（GDB 符号 EMK1，别名 ELKL 基序激酶 1 和 MARK2）。这些同工型的特征是存在一个 162 个碱基对的可变外显子，该外显子产生两种形式，一种包含该外显子，另一种则缺乏它。通过 RT-PCR 分析发现，这两种形式在许多选定的细胞系和组织样本中均有共表达。通过 Northern 印迹法显示，人 EMK1 由一种普遍表达的单一 mRNA 编码。我们根据数据库中保存的两个人类 11 号染色体黏粒的序列重建了 EMK1 的大部分基因组结构，并表明该基因至少由 16 个小外显子组成。
