Xu W, Charles I G, Liu L, Moncada S, Emson P
MRC Molecular Neuroscience Group, Department of Neurobiology, Babraham Institute, Cambridge, UK.
Biochem Biophys Res Commun. 1996 Feb 27;219(3):784-8. doi: 10.1006/bbrc.1996.0311.
Previously, we reported the isolation and molecular cloning of human inducible nitric oxide synthase gene (NOS2) sequences from human chromosome 17 cosmid libraries. Here we describe the further characterization and sequencing of the NOS2 gene. The genomic structure of the NOS2 gene was determined from two overlapping cosmid clones, namely, pcos4A and pcos20. the NOS2 open reading frame is encoded by 27 exons, with translation initiation and termination in exon 2 and exon 27, respectively. These results differ from the previously reported organization of the iNOS gene, where 26 exons were reported for the genomic structure of NOS2.
此前，我们报道了从人类17号染色体黏粒文库中分离和分子克隆人诱导型一氧化氮合酶基因（NOS2）序列。在此，我们描述了NOS2基因的进一步特征分析和测序。NOS2基因的基因组结构由两个重叠的黏粒克隆确定，即pcos4A和pcos20。NOS2开放阅读框由27个外显子编码，翻译起始和终止分别在外显子2和外显子27。这些结果与先前报道的诱导型一氧化氮合酶（iNOS）基因的结构不同，先前报道的NOS2基因组结构有26个外显子。
