Manilal S, Nguyen T M, Morris G E
MRIC Biochemistry Group, N.E. Wales Institute, Wrexham, United Kingdom.
Biochem Biophys Res Commun. 1998 Aug 28;249(3):643-7. doi: 10.1006/bbrc.1998.9209.
Emerin is a nuclear membrane protein which is affected by mutation in X-linked Emery-Dreifuss muscular dystrophy. We have previously suggested that emerin is a member of a family of type II integral membrane proteins which associate with the nuclear lamina and which include lamina-associated proteins and the lamin B receptor. We now show that emerin in COS cells is not restricted to the nuclear rim but is also found at intranuclear sites, where it colocalizes with nuclear lamins B1, B2 and A/C. During mitosis, emerin is dispersed throughout the cell and then participates in the reconstitution of membranes around the daughter nuclei. Although emerin and lamins do not remain colocalized during mitosis, they all show some association with the midbody of the mitotic spindle.
Emerin是一种核膜蛋白，在X连锁的埃默里-德赖富斯肌营养不良症中会因突变而受到影响。我们之前曾提出，Emerin是II型整合膜蛋白家族的成员，该家族与核纤层相关，包括核纤层相关蛋白和核纤层B受体。我们现在发现，COS细胞中的Emerin不仅局限于核边缘，还存在于核内位点，在那里它与核纤层蛋白B1、B2和A/C共定位。在有丝分裂期间，Emerin分散在整个细胞中，然后参与子核周围膜的重建。虽然Emerin和核纤层蛋白在有丝分裂期间不会保持共定位，但它们都与有丝分裂纺锤体的中间体有一定关联。
