Zhong L, Ou J, Barkai U, Mao J F, Frasor J, Gibori G
Department of Physiology and Biophysics, University of Illinois at Chicago 60612, USA.
Biochem Biophys Res Commun. 1998 Aug 28;249(3):797-803. doi: 10.1006/bbrc.1998.9229.
The rat 20 alpha-hydroxysteroid dehydrogenase (20 alpha-HSD) is an enzyme responsible for the catabolism of progesterone to the inactive 20 alpha-hydroxprogesterone. We have previously shown that the expression of this enzyme is not regulated by post-translational modification, but at the level of transcription. In this study we have established that the 20 alpha-HSD gene contains nine exons and have isolated a 2.5 kb promoter region. The transcription start site was identified and a TATA box was found. 5' deletions of this promoter significantly decreased basal promoter activity. Treatment with forskolin led to a dose dependent inhibition of the 2.5kg-20 alpha-HSD-luciferase construct. Computer analysis identified one CRE, two Nur77 response elements, two putative AP1 sites and one progesterone response element half-site. In summary, we have identified and partially characterized the promoter region of the rat ovarian 20 alpha-HSD and demonstrated that the regulatory elements for 20 alpha-HSD are present within a 2.5 kb 5' flanking region of the gene.
大鼠20α-羟基类固醇脱氢酶（20α-HSD）是一种负责将孕酮分解代谢为无活性的20α-羟孕酮的酶。我们之前已经表明，这种酶的表达不受翻译后修饰的调控，而是在转录水平上调控。在本研究中，我们确定20α-HSD基因包含9个外显子，并分离出一个2.5kb的启动子区域。确定了转录起始位点并发现了一个TATA框。该启动子的5'端缺失显著降低了基础启动子活性。用福司可林处理导致对2.5kg-20α-HSD-荧光素酶构建体的剂量依赖性抑制。计算机分析确定了一个CRE、两个Nur77反应元件、两个假定的AP1位点和一个孕酮反应元件半位点。总之，我们已经鉴定并部分表征了大鼠卵巢20α-HSD的启动子区域，并证明20α-HSD的调控元件存在于该基因5'侧翼区域的2.5kb范围内。
