Mouithys-Mickalad A, Hans P, Deby-Dupont G, Hoebeke M, Deby C, Lamy M
Centre for the Biochemistry of Oxygen, University of Liège, Belgium.
Biochem Biophys Res Commun. 1998 Aug 28;249(3):833-7. doi: 10.1006/bbrc.1998.9235.
Peroxynitrite (ONOO-), resulting from the reaction of nitric oxide with superoxide anion, is a powerful oxidant produced in activated macrophages, during ischemia-reperfusion processes as well as in neurodegenerative disorders. This study investigated the reaction of the anesthetic agent propofol (PPF) with ONOO-, using electron spin resonance (ESR) and UV-visible spectrometry. Peroxynitrite was synthetized either from acidified hydrogen peroxide and nitrite, or from sodium azide and ozone. The addition of ONOO- to PPF in alkaline solution (pH 12) allowed to detect a, short lifetime, ESR signal corresponding to a phenoxyl radical. This finding was confirmed by a UV-visible study, resulting in the appearance of 427 nm peak and the disappearance of the peak located at 239 nm. The 291 nm peak remained unchanged. The identification of the end-product of the reaction of PPF with ONOO- needs further investigations.
过氧亚硝酸盐（ONOO-）由一氧化氮与超氧阴离子反应产生，是活化巨噬细胞、缺血再灌注过程以及神经退行性疾病中产生的一种强氧化剂。本研究利用电子自旋共振（ESR）和紫外可见光谱法，研究了麻醉剂丙泊酚（PPF）与ONOO-的反应。过氧亚硝酸盐可由酸化过氧化氢和亚硝酸盐合成，也可由叠氮化钠和臭氧合成。在碱性溶液（pH 12）中向PPF添加ONOO-，可检测到对应于苯氧自由基的短寿命ESR信号。紫外可见研究证实了这一发现，结果出现了427 nm峰，位于239 nm处的峰消失。291 nm峰保持不变。PPF与ONOO-反应终产物的鉴定需要进一步研究。
