Nadkarni G D, Sawant B U
Free Radical Biochemistry Section, Bhabha Atomic Research Centre, Tata Memorial Centre Annexe, Parel, Mumbai.
Indian J Exp Biol. 1998 Jun;36(6):615-7.
Antioxidant defences consisting of superoxide dismutase (SOD), catalase, glutathione peroxidase (GPx), and non-protein thiol (GSH) were estimated in the surviving kidney of unilaterally nephrectomized rats on days 1, 2, 3 and 12, following surgery. Simultaneously renal tissue lipid peroxidation (LP) and plasma GPx was monitored. The aim of this study was to evaluate the role of antioxidant defence in renal pathophysiology. Significant elevations in renal LP, GPx and GSH were evident on the first day and were maintained till 3rd day following nephrectomy. The response of SOD and catalase was slower, attaining significant elevation on 2nd and 3rd day respectively. Tissue GPx was the only parameter which remained elevated till 12th day, while all the other parameters returned to control levels by day 12. Plasma GPx declined significantly on days 3 (13.2%) and 12 (19.6%). These changes appear to be a part of the process when functions of 2 kidneys are taken over by a single kidney following unilateral nephrectomy.
在单侧肾切除术后第1、2、3和12天，对单侧肾切除大鼠存活肾中的抗氧化防御系统进行了评估，该系统由超氧化物歧化酶（SOD）、过氧化氢酶、谷胱甘肽过氧化物酶（GPx）和非蛋白硫醇（GSH）组成。同时监测肾组织脂质过氧化（LP）和血浆GPx。本研究的目的是评估抗氧化防御在肾脏病理生理学中的作用。肾LP、GPx和GSH在第一天显著升高，并在肾切除术后维持到第3天。SOD和过氧化氢酶的反应较慢，分别在第2天和第3天显著升高。组织GPx是唯一直到第12天仍保持升高的参数，而所有其他参数在第12天恢复到对照水平。血浆GPx在第3天（13.2%）和第12天（19.6%）显著下降。这些变化似乎是单侧肾切除后单肾接管双肾功能过程的一部分。
