de Manzoni G, Guglielmi A, Verlato G, Tomezzoli A, Pelosi G, Schiavon I, Cordiano C
Istituto di Semeiotica Chirurgica, Università di Verona, Verona, Italia.
Oncology. 1998 Sep-Oct;55(5):456-60. doi: 10.1159/000011895.
The ability of cancer cells to attach to laminin has been correlated with their metastatic potential and highly metastatic cancer cells seem to express on their surface significantly more laminin receptors than do their much less metastatic or benign counterparts. The expression of 67-kDa laminin receptors (LR) was investigated in a group of 75 patients who underwent gastrectomy for advanced gastric cancer, with special reference to the possible role in the tumor progression and in the overall survival. The tumor LR expression was immunohistochemically determined in paraffin-embedded sections using the MLuC5 monoclonal antibody which recognizes the 67-kDa LR and the avidin-biotin immunoperoxidase method. Of the 75 cases analyzed, 43 cases (57.3%) displayed a positive reaction. The cumulative 5-year survival rate was 72.6% (95% CI 52.5-85.3) for patients without expression of LR and 46.6% (29.8-61.8) for those with positive LR expression. A significant association between LR expression and depth of tumor invasion (0.022) was found. By univariate analysis the presence of laminin receptors seemed to be associated with a higher risk of death [RR 1.72 (95% CI 0.71-4.20], but this effect disappeared after controlling for depth of tumor invasion. In conclusion, these results suggest that tumor expression of laminin receptors could be correlated with tumor aggressiveness. However, the prognostic significance of laminin receptor expression is already provided by the depth of tumor invasion.
癌细胞附着于层粘连蛋白的能力与其转移潜能相关，高度转移的癌细胞表面表达的层粘连蛋白受体似乎比转移能力弱得多或良性的对应细胞显著更多。对75例因进展期胃癌接受胃切除术的患者进行了67-kDa层粘连蛋白受体（LR）表达的研究，特别关注其在肿瘤进展和总生存中的可能作用。使用识别67-kDa LR的MLuC5单克隆抗体及抗生物素蛋白-生物素免疫过氧化物酶法，对石蜡包埋切片进行免疫组织化学测定肿瘤LR表达。在分析的75例病例中，43例（57.3%）呈阳性反应。LR无表达患者的累积5年生存率为72.6%（95%CI 52.5 - 85.3），LR表达阳性患者为46.6%（29.8 - 61.8）。发现LR表达与肿瘤浸润深度之间存在显著关联（0.022）。单因素分析显示，层粘连蛋白受体的存在似乎与较高的死亡风险相关[相对风险1.72（95%CI 0.71 - 4.20）]，但在控制肿瘤浸润深度后，这种效应消失。总之，这些结果表明层粘连蛋白受体的肿瘤表达可能与肿瘤侵袭性相关。然而，肿瘤浸润深度已提供了层粘连蛋白受体表达的预后意义。
