Kautsky M P, Hagerman D D
Steroids. 1976 Aug;28(2):247-59. doi: 10.1016/0039-128x(76)90113-6.
Testosterone, 4-androstene-3,17-dione, 19-hydroxy-4-androstene-3,17-dione, 4alpha-5-oxido-5alpha-androstane-3,17-dione and 4beta-5-oxido-5beta-androstane-3,17-dione were compared as substrates for aromatization by the small particle fraction from sow ovaries. Relative conversion rates were: 19-hydroxy-4-androstene-3,17-dione:4-androstene-3,17-dione: testosterone: 4alpha-5-oxido-5alpha- and 4beta-5-oxido-5beta-androstane-3,17-dione; 100:32:20:congruent to O. Apparent Michaelis constants were 4.4 muM for 4-androstene-3,17-dione and 12 muM for testosterone. Maximum velocities were 0.20 pmoles/mg protein per min for androstenedione [1] and 0.16 for testosterone. The substrate preferences of the aromatizing system found in ovary are similar to those of the enzyme found in placenta.
对睾酮、4-雄烯-3,17-二酮、19-羟基-4-雄烯-3,17-二酮、4α-5-氧化-5α-雄甾烷-3,17-二酮和4β-5-氧化-5β-雄甾烷-3,17-二酮作为母猪卵巢小颗粒组分芳香化作用底物进行了比较。相对转化率为：19-羟基-4-雄烯-3,17-二酮：4-雄烯-3,17-二酮：睾酮：4α-5-氧化-5α-和4β-5-氧化-5β-雄甾烷-3,17-二酮；100:32:20:约为0。4-雄烯-3,17-二酮的表观米氏常数为4.4μM，睾酮为12μM。雄烯二酮的最大反应速度为0.20皮摩尔/毫克蛋白每分钟[1]，睾酮为0.16。卵巢中发现的芳香化系统的底物偏好与胎盘中发现的酶的底物偏好相似。
