Silva A, Fando R, Benitez J A
Facultad de Biología de la Universidad de La Habana, La Habana, Cuba.
Curr Microbiol. 1998 Oct;37(4):231-5. doi: 10.1007/s002849900370.
A mutant cholera toxin B subunit containing a G33E substitution was constructed and expressed in V. cholerae. The G33E amino acid substitution did not affect the amount of recombinant CTB secreted to the culture medium. The overexpression of the mutant B subunits in wild-type toxigenic cholera vibrios led to an 80% decrease in production of active cholera toxin in vitro and in vivo. Overexpression of BG33E subunits could be instrumental in the increase of the biosafety of live attenuated cholera candidate vaccine strains.
构建了一种含有G33E替换的突变型霍乱毒素B亚基，并在霍乱弧菌中进行表达。G33E氨基酸替换不影响分泌到培养基中的重组CTB的量。在野生型产毒素霍乱弧菌中突变B亚基的过表达导致体外和体内活性霍乱毒素产量降低80%。BG33E亚基的过表达可能有助于提高减毒活霍乱候选疫苗株的生物安全性。
