Lebens M, Johansson S, Osek J, Lindblad M, Holmgren J
University of Göteborg, Dept. of Medical Microbiology and Immunology, Sweden.
Biotechnology (N Y). 1993 Dec;11(13):1574-8. doi: 10.1038/nbt1293-1574.
By systematically manipulating promoter and ribosome binding structures, plasmid copy number and the structure of the cholera toxin B (CTB) subunit gene, we were able to develop a plasmid expression system that, when used in conjunction with an optimized growth medium, provided yields of CTB approaching one gram per liter. The CTB protein which was secreted to > 95%, could readily be purified from the growth medium of a V. cholerae production strain and was shown to be immunologically indistinguishable from previously used vaccine preparations of native or recombinant CTB.
通过系统地操纵启动子和核糖体结合结构、质粒拷贝数以及霍乱毒素B（CTB）亚基基因的结构，我们得以开发出一种质粒表达系统。该系统与优化的生长培养基结合使用时，CTB的产量接近每升1克。分泌率大于95%的CTB蛋白能够很容易地从霍乱弧菌生产菌株的生长培养基中纯化出来，并且在免疫学上与先前使用的天然或重组CTB疫苗制剂没有区别。
