Wu JT, Kuo-Huang LL, Lee J
Institute of Botany, Academia Sinica, Nankang, Taipei 115, Taiwan.
Curr Microbiol. 1998 Oct;37(4):257-61. doi: 10.1007/s002849900375.
Peridinium bipes exerted an inhibitory effect on the growth of Microcystis aeruginosa. The algicidal action of water-soluble extract from P. bipes was studied. After treatment with P. bipes extract, the absorption spectrum of M. aeruginosa culture changed markedly, particularly in the ranges of 500 approximately 650 and 420 approximately 460 nm. An increase in absorption in this wavelength resulted from a leakage of phycobilines, both phycocyanin and allophycocyanin, from the treated cells. However, no leakage of chlorophyll was detected. The leakage of phycobilines was a short-term effect, detectable within 1 h of incubation. Both the plasmalemma and thylakoid membranes in M. aeruginosa cells were damaged and distorted in fine structure after treatment with P. bipes extract. It is assumed that algicide from P. bipes exerts its effect on the cell membranes, giving rise to changes in membrane permeability and a dissociation of phycobiline assemblages, but not of chlorophyll complex, on the thylakoid membranes. As a result, phycobilines were leaked out from the cells.
双足多甲藻对铜绿微囊藻的生长具有抑制作用。研究了双足多甲藻水溶性提取物的杀藻作用。用双足多甲藻提取物处理后，铜绿微囊藻培养物的吸收光谱发生了明显变化，特别是在500至650纳米以及420至460纳米范围内。该波长下吸收的增加是由于经处理的细胞中藻胆素（包括藻蓝蛋白和别藻蓝蛋白）的泄漏所致。然而，未检测到叶绿素的泄漏。藻胆素的泄漏是一种短期效应，在孵育1小时内即可检测到。用双足多甲藻提取物处理后，铜绿微囊藻细胞中的质膜和类囊体膜均受到损伤，结构出现扭曲。推测双足多甲藻的杀藻剂作用于细胞膜，导致膜通透性改变以及类囊体膜上藻胆素组合而非叶绿素复合物的解离。结果，藻胆素从细胞中泄漏出来。
