Guerrieri F, Kalous M, Adorisio E, Turturro N, Santoro G, Drahota Z, Cantatore P
Institute of Medical Biochemistry and Chemistry and Center for the Study of Mitochondria and Energy Metabolism (CNR), University of Bari, Italy.
J Bioenerg Biomembr. 1998 Jun;30(3):269-76. doi: 10.1023/a:1020548904384.
In liver mitochondria isolated from hypothyroid rats, the rate of ATP synthesis is lower than in mitochondria from normal rats. Oligomycin-sensitive ATP hydrolase activity and passive proton permeability were significantly lower in submitochondrial particles from hypothyroid rats compared to those isolated from normal rats. In mitochondria from hypothyroid rats, the changes in catalytic activities of F0F1-ATP synthase are accompanied by a decrease in the amount of immunodetected beta-F1, F0 1-PVP, and OSCP subunits of the complex. Northern blot hybridization shows a decrease in the relative cytosolic content of mRNA for beta-F1 subunit in liver of hypothyroid rats. Administration of 3,5,3'-triodo-L-thyronine to the hypothyroid rats tends to remedy the functional and structural defects of F0F1-ATP synthase observed in the hypothyroid rats. The results obtained indicate that hypothyroidism leads to a decreased expression of F0F1-ATP synthase complex in liver mitochondria and this contributes to the decrease of the efficiency of oxidative phosphorylation.
在从甲状腺功能减退大鼠分离出的肝脏线粒体中，ATP合成速率低于正常大鼠的线粒体。与从正常大鼠分离的亚线粒体颗粒相比，甲状腺功能减退大鼠的亚线粒体颗粒中对寡霉素敏感的ATP水解酶活性和质子被动通透性显著降低。在甲状腺功能减退大鼠的线粒体中，F0F1 - ATP合酶催化活性的变化伴随着该复合物免疫检测到的β - F1、F0 1 - PVP和OSCP亚基数量的减少。Northern印迹杂交显示甲状腺功能减退大鼠肝脏中β - F1亚基的mRNA相对胞质含量降低。给甲状腺功能减退大鼠施用3,5,3'-三碘 - L - 甲状腺原氨酸倾向于纠正甲状腺功能减退大鼠中观察到的F0F1 - ATP合酶的功能和结构缺陷。所得结果表明，甲状腺功能减退导致肝脏线粒体中F0F1 - ATP合酶复合物表达降低，这导致氧化磷酸化效率下降。
