肝细胞生长因子在体外刺激胎儿胃上皮细胞生长。

Hepatocyte growth factor stimulates fetal gastric epithelial cell growth in vitro.

作者信息

Kong W, Yee L F, Mulvihill S J

机构信息

Department of Surgery, University of California at San Francisco, 533 Parnassus Avenue, San Francisco, California, 94143-0788, USA.

出版信息

J Surg Res. 1998 Aug;78(2):161-8. doi: 10.1006/jsre.1997.5230.

DOI:10.1006/jsre.1997.5230

PMID:9733635

Abstract

BACKGROUND

The growth and development of the fetal gastrointestinal tract is likely mediated, in part, by peptide growth factors. We compared the mitogenic effects of graded doses of hepatocyte growth factor (HGF) to epidermal growth factor (EGF), transforming growth factor-alpha (TGF-alpha), and insulin-like growth factor-1 (IGF-1) on fetal rabbit gastric epithelial cells.

MATERIALS AND METHODS

Fetal rabbit gastric epithelial cells were purified by mechanical dissociation and selected culture and grown in short-term (24 h) and long-term (12 days) culture. Stimulation of fetal gastric epithelial cell growth in response to individual peptide growth factors was measured by [3H]thymidine incorporation and cell counting.

RESULTS

In short-term culture, HGF stimulated [3H]thymidine incorporation in a dose-dependent manner from a threshold at 10 pM to a maximum at 100 pM. For EGF and TGF-alpha, maximal stimulation occurred at 100 pM. For HGF, maximal [3H]thymidine incorporation was 3.6 +/- 0.7 times basal. For EGF and TGF-alpha, maximal [3H]thymidine incorporation was 4.3 +/- 0.4, and 3.6 +/- 0.4 times basal, respectively. For IGF-1, maximal [3H]thymidine incorporation was only 70% of the maximal effect observed for the other growth factors tested. Rabbit amniotic fluid increased [3H]thymidine uptake in a dose-dependent manner. In long-term culture, purification to greater than 90% epithelial cells was attained after 12 days treatment. For HGF, EGF, TGF-alpha, and 20% rabbit amniotic fluid, significant increases in cell number above control (P < 0.05) were observed at 1 nM concentrations. None of these individual factors, however, increased cell growth as significantly as that of 10% fetal bovine serum.

CONCLUSIONS

Our results suggest that: (1) HGF stimulates [3H]thymidine uptake and cell proliferation in fetal rabbit gastric epithelial cells in vitro, and (2) HGF's mitogenic effect on fetal rabbit gastric epithelial cell growth is comparable to that observed for EGF and TGF-alpha, but superior to the effect observed for IGF-1.

摘要

背景

胎儿胃肠道的生长发育可能部分由肽生长因子介导。我们比较了不同剂量的肝细胞生长因子（HGF）与表皮生长因子（EGF）、转化生长因子-α（TGF-α）和胰岛素样生长因子-1（IGF-1）对胎兔胃上皮细胞的促有丝分裂作用。

材料与方法

通过机械解离和选择培养纯化胎兔胃上皮细胞，并进行短期（24小时）和长期（12天）培养。通过[3H]胸苷掺入和细胞计数来测量单个肽生长因子对胎兔胃上皮细胞生长的刺激作用。

结果

在短期培养中，HGF以剂量依赖性方式刺激[3H]胸苷掺入，从10 pM的阈值到100 pM的最大值。对于EGF和TGF-α，最大刺激发生在100 pM。对于HGF，最大[3H]胸苷掺入量是基础值的3.6±0.7倍。对于EGF和TGF-α，最大[3H]胸苷掺入量分别是基础值的4.3±0.4倍和3.6±0.4倍。对于IGF-1，最大[3H]胸苷掺入量仅为其他测试生长因子最大效应的70%。兔羊水以剂量依赖性方式增加[3H]胸苷摄取。在长期培养中，经过12天处理后，上皮细胞纯化率达到90%以上。对于HGF、EGF、TGF-α和20%的兔羊水，在1 nM浓度下观察到细胞数量比对照有显著增加（P<0.05）。然而，这些单一因子中没有一个能像10%胎牛血清那样显著增加细胞生长。