Ishikawa Takeshi, Ando Takashi, Obayashi Hiroshi, Nakabe Nami, Okita Mika, Isozaki Yutaka, Nagao Yasuyuki, Oyamada Hirokazu, Nakajima Yoshihiro, Kato Haruki, Kokura Satoshi, Naito Yuji, Yoshida Norimasa, Yoshikawa Toshikazu
Department of Gastroenterology, Matsushita Memorial Hospital, 5-55 Sotojima-cho, Moriguchi City, Osaka 570-8540, Japan.
Dig Dis Sci. 2008 Jul;53(7):1785-91. doi: 10.1007/s10620-007-0070-4. Epub 2007 Nov 8.
Ménétrier's disease has been reported to be associated with Helicobacter pylori infection. The aim of this study was to investigate the genetic characteristics of various virulence factors and cytokine expression profiles in Helicobacter pylori isolated from patients with Ménétrier's disease. The genotyping of virulence factors was accomplished by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Induction of various cytokines in MKN45 cells or gastric fibroblasts by Helicobacter pylori stimulus was measured by real-time reverse transcription-PCR. We found that the Helicobacter pylori strain isolated from a patient with Ménétrier's disease was different from other strains in the MseI-RFLP pattern of the ureC gene. Helicobacter pylori isolated from a patient with Ménétrier's disease showed the highest hepatocyte growth factor (HGF) and TNF-alpha mRNA expressions from gastric fibroblasts, and the highest TNF-alpha expression from MKN45 cells. The results in this study suggest that the difference in cytokine production, depending on the difference in bacteria components, plays an important role in the development of Ménétrier's disease.
据报道，门脉性胃病与幽门螺杆菌感染有关。本研究的目的是调查从门脉性胃病患者分离出的幽门螺杆菌中各种毒力因子的遗传特征和细胞因子表达谱。毒力因子的基因分型通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）完成。通过实时逆转录PCR测定幽门螺杆菌刺激MKN45细胞或胃成纤维细胞中各种细胞因子的诱导情况。我们发现，从门脉性胃病患者分离出的幽门螺杆菌菌株在ureC基因的MseI-RFLP模式上与其他菌株不同。从门脉性胃病患者分离出的幽门螺杆菌在胃成纤维细胞中显示出最高的肝细胞生长因子（HGF）和TNF-α mRNA表达，在MKN45细胞中显示出最高的TNF-α表达。本研究结果表明，细胞因子产生的差异取决于细菌成分的差异，在门脉性胃病的发展中起重要作用。
