由M1基因编码的呼肠孤病毒蛋白μ2是一种RNA结合蛋白。
The reovirus protein mu2, encoded by the M1 gene, is an RNA-binding protein.
作者信息
Brentano L, Noah D L, Brown E G, Sherry B
机构信息
Department of Microbiology, Pathology, and Parasitology, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina 27606, USA.
出版信息
J Virol. 1998 Oct;72(10):8354-7. doi: 10.1128/JVI.72.10.8354-8357.1998.
Abstract
The reovirus M1, L1, and L2 genes encode proteins found at each vertex of the viral core and are likely to form a structural unit involved in RNA synthesis. Genetic analyses have implicated the M1 gene in viral RNA synthesis and core nucleoside triphosphatase activity, but there have been no direct biochemical studies of mu2 function. Here, we expressed mu2 in vitro and assessed its RNA-binding activity. The expressed mu2 binds both poly(I-C)- and poly(U)-Sepharose, and binding activity is greater in Mn2+ than in Mg2+. Heterologous RNA competes for mu2 binding to reovirus RNA transcripts as effectively as homologous reovirus RNA does, providing no evidence for sequence-specific RNA binding by mu2. Protein mu2 is now the sixth reovirus protein demonstrated to have RNA-binding activity.
摘要
呼肠孤病毒的M1、L1和L2基因编码在病毒核心每个顶点发现的蛋白质，并且可能形成参与RNA合成的结构单元。遗传分析表明M1基因参与病毒RNA合成和核心核苷三磷酸酶活性，但尚未有对μ2功能的直接生化研究。在此，我们在体外表达了μ2并评估了其RNA结合活性。表达的μ2能结合聚（I-C）-琼脂糖和聚（U）-琼脂糖，并且在Mn2+存在时的结合活性比在Mg2+存在时更强。异源RNA与同源呼肠孤病毒RNA一样有效地竞争μ2与呼肠孤病毒RNA转录本的结合，这没有提供μ2进行序列特异性RNA结合的证据。蛋白质μ2现在是第六种被证明具有RNA结合活性的呼肠孤病毒蛋白。
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本文引用的文献
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Stoichiometry of reovirus structural proteins in virus, ISVP, and core particles.呼肠孤病毒结构蛋白在病毒、中间亚病毒颗粒（ISVP）和核心颗粒中的化学计量。
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Reovirus-induced acute myocarditis in mice correlates with viral RNA synthesis rather than generation of infectious virus in cardiac myocytes.呼肠孤病毒诱导的小鼠急性心肌炎与病毒RNA合成相关，而非与心肌细胞中感染性病毒的产生相关。
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