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人类免疫缺陷病毒1型整合酶在体内对U3和U5附着位点的特异性及独立识别

Specific and independent recognition of U3 and U5 att sites by human immunodeficiency virus type 1 integrase in vivo.

作者信息

Masuda T, Kuroda M J, Harada S

机构信息

Department of Biodefense and Medical Virology, Kumamoto University School of Medicine, Kumamoto, Japan.

出版信息

J Virol. 1998 Oct;72(10):8396-402. doi: 10.1128/JVI.72.10.8396-8402.1998.

DOI:10.1128/JVI.72.10.8396-8402.1998
PMID:9733892
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC110226/
Abstract

The retroviral attachment (att) sites at viral DNA ends are cis-acting regions essential for proviral integration. To investigate the sequence features of att important for human immunodeficiency virus type 1 (HIV-1) integration in vivo, we generated a series of 25 att mutants of HIV-1 by mutagenesis of the U3, U5, or both boundaries of att. Our results indicated that the terminal 11 or 12 bp of viral DNA are sufficient for specific recognition by HIV-1 integrase (IN) and suggested that IN might recognize each att site independently in vivo.

摘要

病毒DNA末端的逆转录病毒附着（att）位点是前病毒整合所必需的顺式作用区域。为了研究对1型人类免疫缺陷病毒（HIV-1）体内整合重要的att序列特征，我们通过对att的U3、U5或两者边界进行诱变，生成了一系列25个HIV-1的att突变体。我们的结果表明，病毒DNA的末端11或12个碱基对足以被HIV-1整合酶（IN）特异性识别，并表明IN可能在体内独立识别每个att位点。