人类免疫缺陷病毒1型整合酶在体内对U3和U5附着位点的特异性及独立识别
Specific and independent recognition of U3 and U5 att sites by human immunodeficiency virus type 1 integrase in vivo.
作者信息
Masuda T, Kuroda M J, Harada S
机构信息
Department of Biodefense and Medical Virology, Kumamoto University School of Medicine, Kumamoto, Japan.
出版信息
J Virol. 1998 Oct;72(10):8396-402. doi: 10.1128/JVI.72.10.8396-8402.1998.
Abstract
The retroviral attachment (att) sites at viral DNA ends are cis-acting regions essential for proviral integration. To investigate the sequence features of att important for human immunodeficiency virus type 1 (HIV-1) integration in vivo, we generated a series of 25 att mutants of HIV-1 by mutagenesis of the U3, U5, or both boundaries of att. Our results indicated that the terminal 11 or 12 bp of viral DNA are sufficient for specific recognition by HIV-1 integrase (IN) and suggested that IN might recognize each att site independently in vivo.
摘要
病毒DNA末端的逆转录病毒附着（att）位点是前病毒整合所必需的顺式作用区域。为了研究对1型人类免疫缺陷病毒（HIV-1）体内整合重要的att序列特征，我们通过对att的U3、U5或两者边界进行诱变，生成了一系列25个HIV-1的att突变体。我们的结果表明，病毒DNA的末端11或12个碱基对足以被HIV-1整合酶（IN）特异性识别，并表明IN可能在体内独立识别每个att位点。
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本文引用的文献
1
Critical contacts between HIV-1 integrase and viral DNA identified by structure-based analysis and photo-crosslinking.通过基于结构的分析和光交联鉴定出的HIV-1整合酶与病毒DNA之间的关键接触点。
EMBO J. 1997 Nov 17;16(22):6849-59. doi: 10.1093/emboj/16.22.6849.
2
A mutation in integrase can compensate for mutations in the simian immunodeficiency virus att site.整合酶中的一个突变可以补偿猿猴免疫缺陷病毒附着位点的突变。
J Virol. 1997 Nov;71(11):8124-32. doi: 10.1128/JVI.71.11.8124-8132.1997.
3
Solution structure of the N-terminal zinc binding domain of HIV-1 integrase.人类免疫缺陷病毒1型整合酶N端锌结合结构域的溶液结构
Nat Struct Biol. 1997 Jul;4(7):567-77. doi: 10.1038/nsb0797-567.
4
The importance of the A-rich loop in human immunodeficiency virus type 1 reverse transcription and infectivity.富含A的环在1型人类免疫缺陷病毒逆转录和感染性中的重要性。
J Virol. 1997 Aug;71(8):5750-7. doi: 10.1128/JVI.71.8.5750-5757.1997.
5
Characterization of feline immunodeficiency virus integrase and analysis of functional domains.猫免疫缺陷病毒整合酶的特性及功能域分析
Virology. 1997 Mar 31;230(1):1-10. doi: 10.1006/viro.1997.8466.
6
Structure-based mutagenesis of the catalytic domain of human immunodeficiency virus type 1 integrase.基于结构的1型人类免疫缺陷病毒整合酶催化结构域诱变
J Virol. 1997 May;71(5):3507-14. doi: 10.1128/JVI.71.5.3507-3514.1997.
7
Central core domain of retroviral integrase is responsible for target site selection.逆转录病毒整合酶的中央核心结构域负责靶位点选择。
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8
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Cell. 1997 Feb 21;88(4):483-92. doi: 10.1016/s0092-8674(00)81888-7.
9
Disruption of the terminal base pairs of retroviral DNA during integration.逆转录病毒DNA整合过程中末端碱基对的破坏。
Genes Dev. 1997 Feb 1;11(3):371-82. doi: 10.1101/gad.11.3.371.
10
Functional identification of nucleotides conferring substrate specificity to retroviral integrase reactions.赋予逆转录病毒整合酶反应底物特异性的核苷酸的功能鉴定。
J Virol. 1997 Feb;71(2):1025-35. doi: 10.1128/JVI.71.2.1025-1035.1997.
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