1型人类免疫缺陷病毒U3区域中体内和体外整合所需的序列。
Sequences in the human immunodeficiency virus type 1 U3 region required for in vivo and in vitro integration.
作者信息
Reicin A S, Kalpana G, Paik S, Marmon S, Goff S
机构信息
Department of Medicine, Columbia College of Physicians and Surgeons, New York, New York 10032, USA.
出版信息
J Virol. 1995 Sep;69(9):5904-7. doi: 10.1128/JVI.69.9.5904-5907.1995.
Abstract
A series of mutants with alterations in the U3 region of the human immunodeficiency virus type 1 long terminal repeat were made, and the effects of these mutations were evaluated both in vitro and in vivo. When the subterminal 6 to 8 nucleotides of the U3 long terminal repeat were mutated, the resulting provirus was unable to efficiently replicate in vivo, and a mutant oligonucleotide which mimicked the mutation could not be efficiently cleaved but could be joined to target DNA by wild-type recombinant integrase protein in vitro. These results suggest that this region is important in the specific recognition of the viral DNA by the integrase protein.
摘要
构建了一系列在人类免疫缺陷病毒1型长末端重复序列U3区域发生改变的突变体，并在体外和体内评估了这些突变的影响。当U3长末端重复序列的末端6至8个核苷酸发生突变时，产生的前病毒在体内无法有效复制，模拟该突变的突变寡核苷酸在体外不能被有效切割，但能被野生型重组整合酶蛋白连接到靶DNA上。这些结果表明，该区域在整合酶蛋白对病毒DNA的特异性识别中很重要。
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