Lee S W, Cho J H, Sung Y C
Department of Life Science, Center for Biofunctional Molecules, School of Environmental Engineering, Pohang University of Science and Technology, Hyoja Dong, Pohang, 790-784 Korea.
J Virol. 1998 Oct;72(10):8430-6. doi: 10.1128/JVI.72.10.8430-8436.1998.
In this study, we have constructed various DNA vaccine vectors that carried hepatitis C virus (HCV) envelope genes without and with the granulocyte-macrophage colony-stimulating factor (GM-CSF) gene in several different ways. In Buffalo rats that received plasmids carrying the HCV envelope genes, which encode envelope proteins E1 and E2, both antibody and lymphoproliferative responses against these proteins were induced. These responses were greatly enhanced by the codelivery of the GM-CSF gene. In particular, inoculation with a bicistronic plasmid that independently expressed the GM-CSF gene and the envelope genes in the same construct generated the highest antibody titers and significantly increased lymphoproliferative responses against these proteins. Moreover, strong antibody responses to homologous and heterologous hypervariable region 1 peptides were elicited in the immunized rats.
在本研究中，我们构建了多种DNA疫苗载体，这些载体以几种不同方式携带丙型肝炎病毒（HCV）包膜基因，有的不带粒细胞巨噬细胞集落刺激因子（GM-CSF）基因，有的带有该基因。在接受携带编码包膜蛋白E1和E2的HCV包膜基因质粒的布法罗大鼠中，诱导了针对这些蛋白的抗体和淋巴细胞增殖反应。GM-CSF基因的共递送极大地增强了这些反应。特别是，接种在同一构建体中独立表达GM-CSF基因和包膜基因的双顺反子质粒产生了最高的抗体滴度，并显著增加了针对这些蛋白的淋巴细胞增殖反应。此外，在免疫大鼠中引发了对同源和异源高变区1肽的强烈抗体反应。
