Greensmith L, Harding D I, Meyer M P, Vrbová G
Department of Anatomy and Developmental Biology, University College London, Gower Street, London WC1E 6BT, UK.
Brain Res Dev Brain Res. 1998 Sep 10;110(1):131-4. doi: 10.1016/s0165-3806(98)00106-0.
During early development, rat soleus muscle fibres are innervated by several axons. Neuromuscular activity is involved in the elimination of all but one terminal, but it is not clear whether electrical or mechanical activity is important. Here, we reduced mechanical activity only, by interfering with excitation-contraction coupling. Muscles treated with dantrolene sodium at 9 days produced significantly less force at 13 days of age than normal muscles, and their sensitivity to ACh was greater than that of controls. The elimination of polyneuronal innervation occurs between days 9-12, but in muscles treated with dantrolene, the loss of synapses was slower. Thus, reducing mechanical activity by interfering with excitation-contraction coupling, (a) delays muscle development and (b) reduces the rate of elimination of polyneuronal innervation.
在早期发育过程中，大鼠比目鱼肌纤维由多条轴突支配。神经肌肉活动参与了除一个终末外所有终末的消除，但尚不清楚电活动或机械活动是否起重要作用。在此，我们仅通过干扰兴奋 - 收缩偶联来降低机械活动。在第9天用丹曲林钠处理的肌肉，在13日龄时产生的力量明显小于正常肌肉，并且它们对乙酰胆碱的敏感性高于对照组。多神经元支配的消除发生在第9 - 12天之间，但在用丹曲林处理的肌肉中，突触的丧失较慢。因此，通过干扰兴奋 - 收缩偶联来降低机械活动，（a）延迟肌肉发育，（b）降低多神经元支配的消除速率。
