Yoon H J, Lee S T, Park Y H
Korean Collection for Type Cultures (KCTC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), Yusong, Taejon, Korea.
Int J Syst Bacteriol. 1998 Jul;48 Pt 3:641-50. doi: 10.1099/00207713-48-3-641.
The 16S-23S internally transcribed spacer (ITS) sequences were analysed to clarify inter- and intraspecific relationships among strains of the genus Nocardioides and the relationship between two Aeromicrobium species. The 16S-23S ITS regions from 33 Nocardioides strains, two Aeromicrobium species and Terrabacter tumescens were sequenced directly after polymerase chain reaction (PCR0 amplification and gamma exonuclease treatment. The genomes of some Nocardioides strains included two types of 16S-23S ITS sequences. The sizes of the 16S-23S ITS sequences of Nocardioides strains ranged from 328 to 539 bp. The 16S-23S ITS sequences of Aeromicrobium erythreum NSP37T, Aeromicrobium fastidiosum NSP38T and T. tumescens NSP39T were 349, 355 and 386 bp long, respectively. Nucleotide similarity among 16S-23S ITS sequences of Nocardioides albus strains and of Nocardioides simplex strains was 84.1-100% and 97.7-100%, respectively. The 16S-23S ITS sequence of Nocardioides luteus was identical to that of 'Nocardioides fulvus' NSP32T and was only 1 bp different from that of 'Nocardioides flavus' strains. However, the 16S-23S ITS sequences of 'N. fulvus' NSP33 showed only a low degree of similarity to 'N. fulvus' NSP32T (54.8%). The degree of 16S-23S ITS similarity between N. luteus NSP20T and N. ablus strains ranged from 85 to 93%. The mean nucleotide similarity values between the type strains of validly described Nocardioides species were highly divergent at 68:1 +/- 16.8%. The two Aeromicrobium species showed a level of 16S-23S ITS similarity of 71.2%. In this study, 16S-23S ITS sequences of the members of the genera Nocardioides and Aeromicrobium were useful for inferring the relationships between closely related strains and species. However, they were not found to be appropriate for elucidating the phylogenetic relationships between distantly related organisms at the genus level.
分析16S - 23S内转录间隔区（ITS）序列，以阐明诺卡氏菌属菌株间的种间和种内关系，以及两种嗜气微菌属物种之间的关系。对33株诺卡氏菌、两种嗜气微菌属物种和肿胀地杆菌的16S - 23S ITS区域进行聚合酶链反应（PCR）扩增和γ核酸外切酶处理后直接测序。一些诺卡氏菌菌株的基因组包含两种类型的16S - 23S ITS序列。诺卡氏菌菌株的16S - 23S ITS序列大小在328至539 bp之间。红嗜气微菌NSP37T、苛求嗜气微菌NSP38T和肿胀地杆菌NSP39T的16S - 23S ITS序列分别长349、355和386 bp。白色诺卡氏菌菌株和简单诺卡氏菌菌株的16S - 23S ITS序列之间的核苷酸相似性分别为84.1% - 100%和97.7% - 100%。淡黄诺卡氏菌的16S - 23S ITS序列与“黄褐诺卡氏菌”NSP32T的相同，与“微黄诺卡氏菌”菌株的序列仅相差1 bp。然而，“黄褐诺卡氏菌”NSP33的16S - 23S ITS序列与“黄褐诺卡氏菌”NSP32T仅显示出低程度的相似性（54.8%）。淡黄诺卡氏菌NSP20T与白色诺卡氏菌菌株之间的16S - 23S ITS相似性程度在85%至93%之间。有效描述的诺卡氏菌属物种的模式菌株之间的平均核苷酸相似性值差异很大，为68.1%±16.8%。两种嗜气微菌属物种的16S - 23S ITS相似性水平为71.2%。在本研究中，诺卡氏菌属和嗜气微菌属成员的16S - 23S ITS序列有助于推断密切相关菌株和物种之间的关系。然而，未发现它们适合于阐明属水平上远缘相关生物体之间的系统发育关系。
