DNA测序显示，五株人型支原体分离株的rrnB操纵子中16S rRNA基因的异质性有限。
DNA sequencing reveals limited heterogeneity in the 16S rRNA gene from the rrnB operon among five Mycoplasma hominis isolates.
作者信息
Mygind T, Birkelund S, Christiansen G
机构信息
Department of Medical Microbiology and Immunology, University of Aarhus, Denmark.
出版信息
Int J Syst Bacteriol. 1998 Jul;48 Pt 3:1067-71. doi: 10.1099/00207713-48-3-1067.
To investigate the intraspecies heterogeneity within the 16S rRNA gene of Mycoplasma hominis, five isolates with diverse antigenic profiles, variable/identical P120 hypervariable domains, and different 16S rRNA gene RFLP patterns were analysed. The 16S rRNA gene from the rrnB operon was amplified by PCR and the PCR products were sequenced. Three isolates had identical 16S rRNA sequences and two isolates had sequences that differed from the others by only one nucleotide.
为研究人型支原体16S rRNA基因的种内异质性，分析了五株具有不同抗原谱、可变/相同P120高变区以及不同16S rRNA基因RFLP模式的分离株。通过PCR扩增rrnB操纵子的16S rRNA基因，并对PCR产物进行测序。三株分离株具有相同的16S rRNA序列，两株分离株的序列与其他序列仅相差一个核苷酸。
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