Hamdy R C, Silvestri A, Rivard C H, Ehrlich M
Centre de Recherche, Hôpital Sainte-Justine, Université de Montréal, Québec, Canada.
Ann Chir. 1997;51(8):875-83.
The histology of bone regeneration in cases of limb lengthening was studied in eight adult dogs. Following an osteotomy of the right fibula, an Orthofix (four dogs) or an Ilizarov external fixator (4 dogs) was installed and an osteotomy of the right tibia performed. Lengthening was started seven days after the surgery at the rate and rhythm of 0.5 mm every 12 hours for three weeks and was then followed by compression of 1 mm. The animals were then sacrificed in pairs 3, 6, 9 and 12 weeks after the start of lengthening. Histological evaluation of regenerate bone was performed using hematoxylin and eosin, trichrome and Von Kossa stain (decalcified). New bone at the site of distraction seemed to be formed mostly through intramembranous, and to a lesser extent, through endochondral ossification as evident by the presence of cartilaginous islands within the distraction gap of old specimens. However, these foci of cartilage cells did not have the appearance of growth plates. From the third week of lengthening, collagen fibers were laid down along the longitudinal axis of distraction. Mineralization of these fibers started at the bone ends and during the following weeks, progressed towards the center of the distraction gap. New bone was formed from both the medullary cavity and the periosteum.
对8只成年犬肢体延长病例的骨再生组织学进行了研究。在右侧腓骨截骨术后，安装了Orthofix（4只犬）或Ilizarov外固定架（4只犬），并对右侧胫骨进行截骨。术后7天开始延长，以每12小时0.5毫米的速度和节奏进行3周，然后进行1毫米的加压。然后在延长开始后的3、6、9和12周成对处死动物。使用苏木精和伊红、三色染色和冯·科萨染色（脱钙）对再生骨进行组织学评估。牵张部位的新骨似乎主要通过膜内成骨形成，在较小程度上通过软骨内成骨形成，这在旧标本牵张间隙内存在软骨岛的情况中很明显。然而，这些软骨细胞灶没有生长板的外观。从延长的第三周开始，胶原纤维沿牵张的纵轴排列。这些纤维的矿化从骨端开始，在接下来的几周内，向牵张间隙的中心发展。新骨由骨髓腔和骨膜形成。
