一种使用双胃线虫科物种的新亚培养物和杀线虫试验。

A new subculture and nematocidal assay using a species of diplogastridae.

作者信息

Yanagida J, Matsuhashi R, Watanabe I, Satou T, Koike K, Nikaido T, Akao S

机构信息

School of Pharmaceutical Sciences, Toho University, Chiba, Japan.

出版信息

Chem Pharm Bull (Tokyo). 1998 Aug;46(8):1261-4. doi: 10.1248/cpb.46.1261.

DOI:10.1248/cpb.46.1261

PMID:9734313

Abstract

A new subculture method and a novel microplate assay for nematocidal activity using a species of Diplogastridae have been developed. The assay gives results rapidly, with high sensitivity in 4 h, and indicated good correlation between action mechanism and the nematode shape when examining 15 known compounds, including the antiparasitic avermectin, antimalarial quinine, and the gamma-amino-n-butyric acidA (GABAA) activated Cl- channel antagonist picrotoxinin. Thus new assay could be used as a primary screening method for new nematocidal compounds.

摘要

已开发出一种新的亚培养方法和一种使用双胃线虫科物种的新型微板杀线虫活性测定法。该测定法结果快速，4小时内具有高灵敏度，并且在检测包括抗寄生虫阿维菌素、抗疟药奎宁和γ-氨基丁酸A（GABAA）激活的氯离子通道拮抗剂印防己毒素在内的15种已知化合物时，表明作用机制与线虫形状之间具有良好的相关性。因此，这种新的测定法可作为新的杀线虫化合物的初步筛选方法。